Keskin, MutluRintamarttunen, JuuliaGülçiçek, EmreRäisänen, Ismo T.Gupta, ShipraTervahartiala, TainaPätilä, TommiSorsa, Timo2023-03-162023-03-162023Keskin, M., Rintamarttunen, J., Gülçiçek, E., Räisänen, I. T., Gupta, S., Tervahartiala, T., Pätilä, T. & Sorsa, T. (2023). A comparative analysis of treatment-related changes in the diagnostic biomarker active metalloproteinase-8 levels in patients with periodontitis. Diagnostics, 13(5), 903.2075-44182075-4418https://hdl.handle.net/20.500.12939/3439Background: Previous studies have revealed the potential diagnostic utility of aMMP-8, an active form of MMP-8, in periodontal and peri-implant diseases. While non-invasive point-of-care (PoC) chairside aMMP-8 tests have shown promise in this regard, there is a dearth of literature on the evaluation of treatment response using these tests. The present study aimed to investigate treatment-related changes in aMMP-8 levels in individuals with Stage III/IV-Grade C periodontitis compared to a healthy control group, using a quantitative chairside PoC aMMP-8 test, and to determine its correlation with clinical parameters. Methods: The study included 27 adult patients (13 smoker, 14 non-smoker) with stage III/IV-grade C periodontitis and 25 healthy adult subjects. Clinical periodontal measurements, real-time PoC aMMP-8, IFMA aMMP-8, and Western immunoblot analyses were performed before and 1 month after anti-infective scaling and root planing periodontal treatment. Time 0 measurements were taken from the healthy control group to test the consistency of the diagnostic test. Results: Both PoC aMMP-8 and IFMA aMMP-8 tests showed a statistically significant decrease in aMMP-8 levels and improvement in periodontal clinical parameters following treatment (p < 0.05). The PoC aMMP-8 test had high diagnostic sensitivity (85.2%) and specificity (100.0%) for periodontitis and was not affected by smoking (p > 0.05). Treatment also reduced MMP-8 immunoreactivity and activation as demonstrated by Western immunoblot analysis. Conclusion: The PoC aMMP-8 test shows promise as a useful tool for the real-time diagnosis and monitoring of periodontal therapy.eninfo:eu-repo/semantics/openAccessDiagnosisInflammationMatrix Metalloproteinase(s)Periodontal DiseasesPeriodontitisArticle1352-s2.0-85149724369Q2WOS:000948143300001Q1