Birant, Sinemİlisulu, Sabiha CerenKılıç, SenemTunca, ZeynepÖzcan, HazalAkkoç, TunçSeymen, Figen2024-05-222024-05-222024Birant, S., İlisulu, S. C., Kılıç, S., Tunca, Z., Özcan, H., Akkoç, T., Seymen, F. (2024). An in vitro assessment of teething gels' effects on human gingival mesenchymal stem cells. BMC Oral Health, 24(1). 10.1186/s12903-024-04213-w1472-6831https://hdl.handle.net/20.500.12939/4699Background: The aim of this study is to examine the cytotoxic effects of dental gels with different contents, which are frequently used during teething, on gingival mesenchymal stem cells (G-MSCs). Method: The teething gels used in this study were Dentinox, Gengigel, Osanite, and Jack and Jill. The human gingival mesenchimal stem cells (hG-MSCs) were incubated with these teething gel solutions (0.1%, 50% and 80% concentrations). Reproductive behavior of G-MSCs was monitored in real time for 72 h using the xCELLigence real-time cell analyzer (RTCA) system. Two-way repeated Anova test and post hoc Bonferroni test were used to evaluate the effect of concentration and dental gel on 0-hour and 72-hour viability. Significance was evaluated at p < 0.05 level. Results: Teething gels prepared at 50% concentration are added to the G-MSC culture, the "cell index" value of G-MSCs to which Dentinox brand gel is added is significantly lower than all other groups (p = 0.05). There is a statistically significant difference between the concentrations in terms of cell index values at the 72nd hour compared to the 0th hour (p = 0.001). Conclusions: The local anesthetic dental gels used in children have a more negative effect on cell viability as concentration increases.eninfo:eu-repo/semantics/openAccessCell viabilityHyaluranic acidLidocainStem cellTeething gelArticle2412-s2.0-85193464677Q2WOS:001227194100003Q1