Hekmatshoar, YaldaKaradağ Gürel, AynurAydoğan, Adem2025-08-142025-08-142025Hekmatshoar, Y., Karadağ Gürel, A., Aydoğan, A. (2025). THE COMPARISON OF MICRORNAS INVOLVED IN SMALL CELL LUNG CANCER AND NON-SMALL CELL LUNG CANCER THROUGH BIOINFORMATICS ANALYSES. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, 49(2), 284-300. 10.33483/jfpau.15290912564-6524https://hdl.handle.net/20.500.12939/5902CODEN : AUEDEObjective: Lung cancer is a major cause of cancer-related deaths worldwide. There are two main types of lung cancer, small cell and non-small cell. Finding new methods for achieving a good prognosis, developing targeted therapy and identifying potential biomarkers is crucial for improving the clinical efficacy of lung cancer. The aim of this study was to investigate the pathogenesis and potential molecular markers by finding differentially expressed miRNAs in 2 subtypes of lung cancer. Materials and Methods: The datasets GSE19945 and GSE135918 containing miRNA data were downloaded from the GEO database. Analyzed with GEO2R online analysis tool with P<0.05 and log fold change |(FC)|≥ 1. Target genes of differentially expressed miRNAs have been identified. Network visualisation and module identification were performed using Cytoscape PPI. Three of the miRNA target genes were selected and validation of the genes was performed in the non-small cell lung cancer cell line A549. Results and discussion: 17 common miRNAs with decreased expression and 2 with increased expression include hsa-miR-1249, hsa-miR-326, hsa-let-7c, hsa-miR-199a-5p, hsa-miR-940, hsamiR-139-3p, hsa-miR-142-3p, hsa-miR-142-5p, hsa-miR-455-5p, hsa-miR-146b-5p,hsa-miR-152hsa-miR-133b, hsa-miR-498, hsa-miR-199b-5p, hsa-miR-140-3p, hsa-miR-203 and hsa-miR-139-5p. Defining the molecular functions and signaling pathways of miRNAs may deepen the current understanding of the molecular mechanisms of the 2 cancer types and contribute to the development of treatment options.eninfo:eu-repo/semantics/openAccessBioinformatics analysislung cancermicroRNAnon-small cell lung cancersmall cell lung cancerArticle10.33483/jfpau.15290914922843002-s2.0-105006679888Q4