Özalp, V. CengizBayramoğlu, GülayArıca, M. Yakup2021-05-152021-05-1520152046-2069https://doi.org/10.1039/c5ra15677ghttps://hdl.handle.net/20.500.12939/627Ozalp, Veli Cengiz/0000-0002-7659-5990Enzyme-magnetic nanoparticle hybrids have been employed as a strategy to improve the biocatalytic usability of biological molecules. For this purpose, we synthesized magnetic core-silica shell nanoparticles for direct one-step easy immobilization of Taq polymerase from a crude extract preparation. The hybrid magnetic particles were synthesized by a magnetic co-precipitation method. The magnetic particles were then coated with a 3-(triethoxysilyl)-propylamine (APTES)/tetraethoxysilane (TEOS) mixture, and the pendant -NH2 groups subsequently functionalized with glutaraldehyde for simultaneous immobilization of Taq-polymerase. The magnetic properties of the particles contributed to fast purification to eliminate inhibitory elements present in the crude extract during Taq polymerase isolation. The Taq-silica hybrid material performed with a similar efficiency to the solution-phase enzyme. Additionally, the new hybrid material allowed reuse of the enzyme multiple times. The silica-Taq polymerase hybrid lost 16% of its activity after 6 cycles. Most importantly, the silica microparticle immobilization extended the functional life of Taq polymerase at room temperature by facilitating a fast cleaning procedure.eninfo:eu-repo/semantics/closedAccessMagnetic SilicaNanoparticle-Taq Polymerase HybridsArticle10.1039/c5ra15677g510687672876782-s2.0-84945312264Q1WOS:000363233500094Q2