Ercal, PınarŞişmanoğlu, Soner2022-03-172022-03-172022Sismanoglu, S., & Ercal, P. (2022). Effects of calcium silicate‐based cements on odonto/osteogenic differentiation potential in mesenchymal stem cells. Australian Endodontic Journal.https://hdl.handle.net/20.500.12939/2284The objective of this study was to evaluate the biological effects and odonto/osteogenic differentiation potential of Biodentine, NeoMTA Plus and TheraCal LC in tooth germ-derived stem cells(TGSCs). TGSCs were exposed to the material extracts. Biocompatibility was tested with MTS cell proliferation assay. Odonto/osteogenic differentiation was assessed with alkaline phosphatase (ALP) activity and mRNA gene expressions (RUNX2, DSPP and DMP-1). Scanning electronic microscopy/ energy-dispersive X-ray (SEM/EDX) analysis and pH analysis were also performed for the materials. Data were evaluated using the one-way ANOVA and Tukey's tests. TGSCs remained viable after 7 days of incubation with all tested materials. Biodentine and NeoMTA Plus showed high ALP activity and increased expression of RUNX2, DSPP and DMP-1 compared to that of TheraCal LC. All materials can induce odonto/osteogenic differentiation of MSCs in various levels. Biocompatibility and odonto/osteogenic differentiation potential of Biodentine and NeoMTA Plus are similar and superior to that of TheraCal LC.eninfo:eu-repo/semantics/closedAccessCalcium SilicatesMesenchymal Stem CellsMesenchymal Stem CellsOdonto/Osteogenic DifferentiationPulp CappingVital Pulp TherapyArticle49166742-s2.0-85125411303Q2WOS:000783032300001Q3