İnal, EbrarNath, Ebru OzdemirAbudayyak, MahmoudUlusoy, Şeymaİnan, Hatice AkbalÇicek, MahmutKartal, Murat2023-09-062023-09-062023İnal, E., Nath, E. Ö., Abudayyak, M., Ulusoy, Ş., İnan, H. A., Çiçek, M., & Kartal, M. (2023). Chemical composition of different parts of the vitex agnus-castus l. essential oils and their in-vitro cytotoxic activities. Records of Natural Products.1307-6167https://hdl.handle.net/20.500.12939/3808The essential oil (EO) compositions and chemotypes of the important ethnomedicinal plant Vitex agnus-castus L. flowers, leaves, and fruits collected from Turkiye (Balikesir and Istanbul) were identified in the present study. Different parts of the V. agnus-castus EO's in-vitro cytotoxic effects on the MCF-7 (human breast adenocarcinoma) and A549 (human lung carcinoma) human-origin cell lines were anaylzed in the current study. The composition of hydrodistiled EOs extracted from flowers, leaves, and fruits of V. agnus-castus were analyzed by GC-FID/MS. Monoterpene hydrocarbons and oxygenated monoterpene compounds were detected as the predominant component class of the V. agnus-castus. EOs extracted from Balikesir region were defined as the "apinene-1,8-cineole" chemotype, while EOs extracted from Istanbul region were defined as the "sabinene-1,8-cineole" chemotype. Sesquiterpene hydrocarbons constituted more than 20% of the compounds in the EOs extracted from the flowers. To the best of our knowledge, this study is the first to analyze the in-vitro cytotoxic effects of flowers. This study is also the first to show the in-vitro cytotoxic effects of fruit, the most commonly used part of the plant, EO on the MCF-7 cell line. Balikesir region's EOs were observed as more potent-especially the purple flower's IC50 is about 4.68 mu g/mL on the MCF7 cell line- than Istanbul regions, which might be attributed to the higher amount of a-pinene, caryophyllene, and limonene content. Our results indicated that the V. agnus-castus EOs, which contain a-pinene, 1,8-cineole, caryophyllene, and limonene as major components, showed relatively high cytotoxic effects compared to the control groups on the MCF7 and A549 cell lines.eninfo:eu-repo/semantics/openAccessVitex agnus-castus L.ChasteberryEssential OilCancerCytotoxicityArticle2-s2.0-85171357676Q2WOS:001033834400001Q3