Taşbaşı, B. BüşraGüner, Buket C.Sudağıdan, MertUçak, SametKavruk, MuratÖzalp, Veli Cengiz2021-05-152021-05-1520190003-26971096-0309https://doi.org/10.1016/j.ab.2019.113449https://hdl.handle.net/20.500.12939/285Ozalp, Veli Cengiz/0000-0002-7659-5990; Kavruk, Murat/0000-0001-5331-7253; UCAK, SAMET/0000-0002-3461-2481Lateral flow assay (LFA) type of biosensors have been popular due to cost-effectiveness and easy-interpretation for instant results, most common examples of applications being pregnancy tests, food safety or medical diagnostics. There are several examples of reports with high sensitivity, including pre-concentration of the sample by magnetic pull-down. However, sensitivity and direct detection designs with aptamers has been a limiting factor for developing aptamers-based LFA assays. In this study, we report a lateral flow design based on aptamer-gated silica nanoparticles to develop high sensitivity and direct bacterial assay by shifting aptamers-target interaction to conjugation pad. Aptamer-gated silica nanoparticles-based biosensors were reported for their high sensitivity, specificity and label-free detection for small molecules and whole cells. This label-free strategy for LFA can determine L. monocytogenes in minced chicken matrix at less than 5 min with a limit of detection (LOD) of 53 cells in one mL samples.eninfo:eu-repo/semantics/closedAccessAptamersListeriaSilicaNanoparticlesPeroxidaseArticle10.1016/j.ab.2019.113449587315574632-s2.0-85072607305Q2WOS:000518177200009Q2