Özer, Nazlı EzerMollaoğlu, Ayça Doğan2024-12-272024-12-272024Özer, N. E., Mollaoğlu, A. D. (2024). Changes in secondary structure of protein in skeletal muscle due to high-carbohydrate of high-fat diets. Meandros Medical and Dental Journal, 25(3), 233-243. 10.69601/meandrosmdj.15379782149-9063https://hdl.handle.net/20.500.12939/5124Objective: Obesity, which arises from changes in lifestyle and feeding habits, poses a threat to human health. One essential contributor to the increase in obesity rates is the popularity of high-calorie diets. This study aims to investigate high-fat (HFD) and high-carbohydrate (HCD) diet-induced molecular changes in protein secondary structure in longissimus dorsi skeletal muscle tissues of female inbred C57BL/6J mice by utilizing Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy. Materials and Methods: Mice were fed a control diet, HCD, or HFD for 24 weeks. Their skeletal muscle tissues were collected, and their spectra were recorded using a Bruker Invenio S ATR-FTIR spectrometer in the 4000-400 cm-1 region. Results: The protein secondary structure profiles of the HCD group demonstrated a significant rise in antiparallel beta-sheet and beta-turn and a decline in parallel beta-sheets, together with the insignificant increase in aggregated beta-sheets and a decrease in alpha-helix. The impact of an HFD on protein conformation is less pronounced than HCD. The HFD diet led to an increase in antiparallel beta-sheets and a decrease in parallel beta sheets. Although insignificant, an increase was observed in beta-turn and alpha-helix. Conclusion: These results propose the appearance of protein aggregation and/or formation of protein- protein intermolecular interaction in skeletal muscle tissues of female inbred C57BL/6J mice. Collectively, these data suggest that both high-calorie diets impair secondary structures of protein in skeletal muscle that may affect its metabolic function.eninfo:eu-repo/semantics/openAccessObesityHigh-carbohydrate dietHigh-fat dietProtein secondary structureFTIRSpectroscopyArticle2532332431267786WOS:001378426300001