Browsing by Author "Arıca, M. Yakup"
Now showing items 1-13 of 13
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A facile and efficient method of enzyme immobilization on silica particles via Michael acceptor film coatings: immobilized catalase in a plug flow reactor
Bayramoğlu, Gülay; Arıca, M. Yakup; Genç, Ayşenur; Özalp, V. Cengiz; İnce, Ahmet; Bıçak, Niyazi (Springer, 2016)A novel method was developed for facile immobilization of enzymes on silica surfaces. Herein, we describe a single-step strategy for generating of reactive double bonds capable of Michael addition on the surfaces of silica ... -
Adsorption and separation of immunoglobulins by novel affinity core-shell beads decorated with protein L and L-histidine
Bayramoğlu, Gülay; Özalp, V. Cengiz; Arıca, M. Yakup (Elsevier Science Bv, 2013)A novel core shell beaded chromatographic materials was prepared by grafting of glycidyl methacrylate (GMA) on to the surface of poly(hydoxypropyl methacrylate/ethyleneglycol dimethacrylate), p(HPMA/EGDMA) beads via ... -
Examination of fabrication conditions of acrylate-based hydrogel formulations for doxorubicin release and efficacy test for hepatocellular carcinoma cell
Bayramoğlu, Gülay; Gözen, Damla; Ersoy, Gözde; Özalp, Veli Cengiz; Akçalı, K. Can; Arıca, M. Yakup (Taylor & Francis Ltd, 2014)The objective of the present study was to develop 2-hydroxypropyl methacrylate-co-polyethylene methacrylate [p(HPMA-co-PEG-MEMA)] hydrogels that are able to efficiently entrap doxorubicin for the application of loco-regional ... -
Fibrous polymer grafted magnetic chitosan beads with strong poly(cation-exchange) groups for single step purification of lysozyme
Bayramoğlu, Gülay; Tekinay, Turgay; Özalp, Veli Cengiz; Arıca, M. Yakup (Elsevier Science Bv, 2015)Lysozyme is an important polypetide used in medical and food applications. We report a novel magnetic strong cation exchange beads for efficient purification of lysozyme from chicken egg white. Magnetic chitosan (MCHT) ... -
Immobilized lipase on micro-porous biosilica for enzymatic transesterification of algal oil
Bayramoğlu, Gülay; Akbulut, Aydın; Özalp, Veli Cengiz; Arıca, M. Yakup (Inst Chemical Engineers, 2015)Enzymatic transesterification reactions for biodiesel production require harsh conditions, which require methods of enzyme stability enhancements. In this study, we present covalently immobilized lipase on the biosilica-polymer ... -
Improvement stability and performance of invertase via immobilization on to silanized and polymer brush grafted magnetic nanoparticles
Bayramoğlu, Gülay; Doz, Tuğce; Özalp, Veli Cengiz; Arıca, M. Yakup (Elsevier Sci Ltd, 2017)In this study, magnetic nanoparticles (Fe3O4) were modified sequentially with silica (Fe3O4@SiO2), glycidyl methacrylate (GMA) by surface initiated atom transfer radical polymerization (SI-ATRP) and hexamethylene diamine ... -
Lysozyme specific aptamer immobilized MCM-41 silicate for single-step purification and quartz crystal microbalance (QCM)-based determination of lysozyme from chicken egg white
Bayramoğlu, Gülay; Özalp, V. Cengiz; Yılmaz, Meltem; Güler, Ulku; Salih, Bekir; Arıca, M. Yakup (Elsevier, 2015)In the present study, MCM-41 silica particles were functionalized with 3-aminopropyltriethoxysilane (APTES) and characterized by FTIR, SEM, TEM and, Brunauer Emmett Teller (BET) analysis. A lysozyme specific aptamer was ... -
Magnetic silica nanoparticle-Taq polymerase hybrids for multiple uses in polymerase chain reaction
Özalp, V. Cengiz; Bayramoğlu, Gülay; Arıca, M. Yakup (Royal Soc Chemistry, 2015)Enzyme-magnetic nanoparticle hybrids have been employed as a strategy to improve the biocatalytic usability of biological molecules. For this purpose, we synthesized magnetic core-silica shell nanoparticles for direct ... -
Pathogen detection by core-shell type aptamer-magnetic preconcentration coupled to real-time PCR
Özalp, Veli Cengiz; Bayramoğlu, Gülay; Kavruk, Murat; Keskin, Batuhan B.; Öktem, Hüseyin A.; Arıca, M. Yakup (Academic Press Inc Elsevier Science, 2014)The presence of pathogenic bacteria is a major health risk factor in food samples and the commercial food supply chain is susceptible to bacterial contamination. Thus, rapid and sensitive identification methods are in ... -
Pathogen detection in complex samples by quartz crystal microbalance sensor coupled to aptamer functionalized core-shell type magnetic separation
Özalp, Veli C.; Bayramoğlu, Gülay; Erdem, Zehra; Arıca, M. Yakup (Elsevier Science Bv, 2015)A quartz crystal microbalance sensor (QCM) was developed for sensitive and specific detection of Salmonella enterica serovar typhimurium cells in food samples by integrating a magnetic bead purification system. Although ... -
Preparation and characterization of mixed-mode magnetic adsorbent with p-amino-benzamidine ligand: Operated in a magnetically stabilized fluidized bed reactor for purification of trypsin from bovine pancreas
Bayramoğlu, Gülay; Özalp, Veli Cengiz; Altıntaş, Begum; Arıca, M. Yakup (Elsevier Sci Ltd, 2014)The magnetic beads were synthesized using glycidylmethacrylate (GMA) and methylmethacrylate (MMA) monomers. A multimodal ligand (i.e., p-amino-benzamidine) was covalently immobilized onto magnetic beads after glutaraldehyde ... -
Removal of disperse red 60 dye from aqueous solution using free and composite fungal biomass of lentinus concinnus
Bayramoğlu, Gülay; Özalp, V. Cengiz; Arıca, M. Yakup (Iwa Publishing, 2017)Lentinus concinnus biomass was immobilized to carboxyl derivative of cellulose, carboxymethyl cellulose (CMC), in the presence of FeCl3 (0.1 mol L-1) via ionic cross-linking. The beads containing immobilized fungal biomass ... -
Staphylococcus aureus detection in blood samples by silica nanoparticle-oligonucleotides conjugates
Borsa, Barış Ata; Tuna, Bilge G.; Hernandez, Frank J.; Hernandez, Luiza I.; Bayramoğlu, Gülay; Arıca, M. Yakup; Özalp, Veli Cengiz (Elsevier Advanced Technology, 2016)A fast, specific and sensitive homogeneous assay for Staphylococcus aureus detection was developed by measuring the activity of secreted nuclease from the bacteria via a modified DNA oligonucleotide. As biosensor format, ...