Browsing by Author "Allahverdiyev, Adil"
Now showing 1 - 2 of 2
Results Per Page
Sort Options
Item Investigation of neurosphere activity of injectable 3D graphene bioink biomaterial(2024) Yıldız, Aslı Pınar Zorba; Yavuz, Burçak; Abamor, Emrah Şefik; Darıcı, Hakan; Allahverdiyev, AdilPurposeThe aim of this study includes the comparative examination of neurosphere formation by WJ-derived mesenchymal stem cells in both 2D media and 3D injectable graphene and graphene-free bioink systems in terms of both immunostaining and gene expression levels.MethodsFor this purpose, hydrogel bioinks were first created and the wj-MKH spheroidal structure was formed on 3D-B (without graphene) and 3D-G (containing graphene). Then, following the differentiation procedure, neurosphere transformations were identified by both immunostaining (b-III Tubulin and Sox2), and Tubulin 3, Sox2, and Nestin markers were examined at the gene expression level with Real-Time PCR, and the results were compared with the 2D environment.ResultsAccording to the results obtained, neurosphere formation occurred more in the 3D environment compared to the 2D environment, obtained both by immunostaining and gene expression levels. It was also observed that differentiation formed neuron-like structures, especially in the 3D-G group containing graphene.ConclusionAs a result, it has been observed that the use of graphene with a non-toxic concentration in the hydrogel injectable system provides better differentiation of stem cells, especially those that will form the cell leg of the biomaterial.Lay SummaryTherefore, the use of graphene-containing hydrogels in injectable systems in nerve damage may increase the effectiveness on nerve regeneration.Item Preparation and characterization of graphene-based 3D biohybrid hydrogel bioink for peripheral neuroengineering(Jove, 2022) Zorba Yıldız, Aslı Pınar; Darıcı, Hakan; Yavuz, Burçak; Abamor, Emrah Şefik; Özdemir, Ceren; Yasin, Müge Elif; Bağırova, Melahat; Allahverdiyev, Adil; Karaöz, ErdalPeripheral neuropathies can occur as a result of axonal damage, and occasionally due to demyelinating diseases. Peripheral nerve damage is a global problem that occurs in 1.5%-5% of emergency patients and may lead to significant job losses. Today, tissue engineering-based approaches, consisting of scaffolds, appropriate cell lines, and biosignals, have become more applicable with the development of three-dimensional (3D) bioprinting technologies. The combination of various hydrogel biomaterials with stem cells, exosomes, or bio-signaling molecules is frequently studied to overcome the existing problems in peripheral nerve regeneration. Accordingly, the production of injectable systems, such as hydrogels, or implantable conduit structures formed by various bioprinting methods has gained importance in peripheral neuro-engineering. Under normal conditions, stem cells are the regenerative cells of the body, and their number and functions do not decrease with time to protect their populations; these are not specialized cells but can differentiate upon appropriate stimulation in response to injury. The stem cell system is under the influence of its microenvironment, called the stem cell niche. In peripheral nerve injuries, especially in neurotmesis, this microenvironment cannot be fully rescued even after surgically binding severed nerve endings together. The composite biomaterials and combined cellular therapies approach increases the functionality and applicability of materials in terms of various properties such as biodegradability, biocompatibility, and processability. Accordingly, this study aims to demonstrate the preparation and use of graphene-based biohybrid hydrogel patterning and to examine the differentiation efficiency of stem cells into nerve cells, which can be an effective solution in nerve regeneration.
