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    Comparative Proteomic Analysis of Dental-Origin Stem Cells: Insights into Regenerative Potential
    (Springer, 2025) Tez, Banu Çiçek; Durukan, Sebahat Melike; Yıldır, Selin Kübra; Çokkeçeci, Murat; Boyvat, Dudu; Altınsoy, Nilay; Fındık, Fatma; Ayaz Güner, Şerife; Acar, Mustafa Burak; Gelderisi, Umberto; Özcan, Servet
    Teeth are a significant source of stem cells and have clinical importance for regenerative medicine. A human tooth harbors different kinds of stem cells in the dental pulp (DPSC) or the periodontal ligament (PDLSC). Also exfoliated teeth in childhood contain a special type of stem cells in their pulp called Stem cells from Human Exfoliated Deciduous teeth (SHED). All these stem cells have features and capacities that vary depending on their niche. Here we investigated the proteomic properties of three types of stem cells that originated from human teeth. We isolated and cultured the DPSCs, PDLSCs, and SHED cells. After validating MSC populations via immunophenotyping, we performed a mass spectrometry-based proteomic approach to identify and relatively quantify whole cell and secreted proteins. Identified proteins were evaluated by using Gene Ontology and Reactome pathway analysis tools. Our data reveal that SHED cells represented inflammation, hypoxia, and nutrient deficiency-associated ontologies in both their secretome and whole-cell proteomes. The whole-cell proteome of PDLSCs consisted of differentiation and proliferation-associated molecules while their secretory molecules were mainly associated with inflammation, ECM organization, and immune response. Among dental-originated stem cells, DPSCs appeared to be the healthiest and clinically relevant in terms of proteomic properties with their proliferation, growth factor signaling, and stemness-associated molecules in their secretome and whole-cell proteome. Obtained results demonstrated that every type of stem cell from dental origin has unique proteomic features that are altered by their location and physiological conditions. The findings may help researchers improve the dental stem-cell-based regenerative medicine approaches.
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    Öğe
    Shear bond strength of composite to demineralized enamel conditioned with resin infiltration
    (2022) Durukan, Sebahat Melike; Gümüştaş, Burak; Sısmanoglu, Soner
    Aim: The aim of this in vitro study was to evaluate the influence of resin infiltration on bond-strength of composite resin to demineralized enamel. Methodology: Thirty bovine incisors were used in this study. Buccal enamel surfaces of bovine incisors were wet polished and then were divided into three groups: sound enamel; demineralized enamel; demineralized enamel infiltrated with a low-viscosity resin (ICON, DMG, Hamburg, Germany). After acid-etching with 37% phosphoric acid for 20 seconds, a two-step, total-etch adhesive (Single Bond 2, 3M ESPE, St. Paul, MN, USA) was applied using a microbrush for 20 seconds, followed by gentle air-drying for 5 seconds. The adhesive was light-cured for 10 seconds. Following the adhesive application, flowable composite resin (Filtek Supreme Flowable, 3M ESPE, St. Paul, MN, USA) was gently placed into a microtubule and was photopolymerized using an LED curing unit (Elipar Deep Cure; 3M ESPE, St. Paul, MN, USA). The microshear bond strength (µSBS) tests were performed using a microshear testing machine at a cross head speed of 0.5 mm/min. One-way ANOVA and Bonferroni tests were used to analyze the data (5%). Results: Significant differences were found according to the ANOVA (p < 0.05). Pair-wise comparison results of µSBS (mean ± SD) were: sound enamel (25.16 ± 2.3); demineralized enamel (17.93 ± 2.1); demineralized enamel infiltrated with a low-viscosity resin (28.51 ± 3.76). Conclusion: Resin infiltration applied to demineralized enamel before composite application increased the bond strength. No difference was found in the bond strength values obtained for sound enamel and resin infiltrated enamel.