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Öğe A comparison of surface roughness values of various restorative materials immersed in pedodontic pre-and probiotics(AMG Transcend Association, 2021) Ozan, Günce; Eren, Meltem Mert; Gürcan, Aliye Tuğce; Bilmez, Zuhal Yıldırım; Yücel, Yasemin YücelThe present in vitro study aims to evaluate pre-and probiotic liquids' effect on surface roughness values of restorative materials after one month of immersion. 360 disc-shaped samples (5mm x 2mm) were prepared from two types of glass-ionomer cements (GIC), a resin-modified GIC, a compomer, three bulk-fill composites, and one microhybrid composite. After the surfaces were polished, samples were divided into three groups (n = 15) and immersed for 10 minutes daily for one month in either a probiotic sachet, kefir (prebiotic), or artificial saliva. After that, the surface roughness values were measured by a profilometer. Scanning electron microscopy (SEM) evaluations of one sample from tested materials were also added. Statistical data were analyzed using one-way ANOVA and Bonferroni–Dunn tests. One of the GIC materials had significantly rougher surfaces in the probiotic sachet, followed by the compomer (p < 0.05). The compomer showed the roughest surfaces after immersion in kefir, followed by the microhybrid composite (Z250). Probiotic sachets formed rougher surfaces than kefir among samples. SEM images revealed the inorganic filler structures and microcracks on the surfaces. A high-viscosity glass-ionomer cement, Equia Fil Forte, and other composite-based materials tested in the present study can be used in pediatric patients who use pre-and probiotic supplements. © 2021 by the authors.Öğe Antimicrobial activity of sapindus mukorossi and saponaria officinalis extracts on streptococcus mutans and enterococcus faecalis(Annals of Medical Research, 2021) Dikmen, Benin; Ozan, GünceAim: Studies on the antimicrobial effectiveness of saponins against oral pathogens are conflicting. Therefore, this study aimed to evaluate the antimicrobial activity of saponins from three different extracts of Sapindus mukorossi and Saponaria officinalis plants against Streptococcus mutans and Enterococcus faecalis. Materials and Methods: The fruit of Sapindus mukorossi and the root of Saponaria officinalis materials were sliced into small pieces and macerated with hexane, ethyl acetate (EtOAc) and methanol (MeOH), in this order. The antimicrobial activity of the extracts against Streptococcus mutans and Enterococcus faecalis was determined by the broth microdilution method based on the minimum inhibitory concentrations (MIC) of each extract. Results: Except for the hexane extract of Saponaria Officinalis, all extracts of both plants had an inhibitory activity against the tested microorganisms. Higher MIC values were obtained against Enterococcus faecalis than Streptococcus Mutans. The lowest MIC values for both Sapindus mukorossi and Saponaria officinalis were against Streptococcus mutans (4 mg/ml). Conclusion: Saponins extracted from Sapindus mukorossi and Saponaria officinalis have a potential antibacterial activity against oral pathogens. They may be used as an ingredient in dental agents.Öğe Color stability of bulk-fill composites immersed in different drinks(Wiley, 2018) Erdemir, Uğur; Kaner, Alev Özsoy; Eren, Meltem Mert; Ozan, Günce; Yıldız, EsraBulk-fill composites show structural differences compared to those of conventional resin composites. Their more reactive photoinitiators, monomer compositions, and increased filler content may result in higher color stability than for conventional ones. Therefore, this study aimed to assess the color stability of three different bulk-fill composites and one conventional resin composite with a higher filler load after 1week and 1month of immersion in different drinks. The resin composite materials that were tested demonstrated significantly different color changes after immersion in the four solutions during both evaluation periods. Immersion in coffee and red wine showed noticeable staining of all the materials. The discoloration effect of staining solutions on the resin composites depends both on the material composition and on the pigment types found in the solutions.Öğe Effect of surface sealant on surface roughness and bacterial adhesion of bulk-fill composites(Sage Publications Ltd, 2021) Ozan, Günce; Eren, Meltem Mert; Vatansever, Cansu; Erdemir, UğurSurface sealants are reported to ensure surface smoothness and improve the surface quality of composite restorations. These sealants should also reduce the bacterial adhesion on composite surfaces however, there is not much information regarding their performance on bulk-fill composite materials. The aim of this study was to evaluate the effect of surface sealant application on surface roughness and bacterial adhesion of various restorative materials. Disc-shaped samples were prepared from a compomer, a conventional composite and three bulk-fill composites. Specimens of each group were divided into two groups (n = 9): with/without surface sealant (Biscover LV, [BLV]). Surface roughness values were examined by profilometry and two samples of each group were examined for bacterial adhesion on a confocal laser scanning microscope (CLSM). Bacterial counts were calculated by both broth cultivation and microscopic images. Results were analyzed with one-way ANOVA and Bonferroni/Dunn tests. Following the BLV application, there was a decrease in the surface roughness values of all groups however, only Tetric N-Ceram Bulk and Beautifil-Bulk groups showed significantly smoother surfaces (p < 0.001). There were no significant differences among material groups without BLV application. Evaluating bacterial adhesion after BLV application, conventional composite had the lowest values among all followed by the compomer group. Beautifil-Bulk had significantly the highest bacterial adhesion (p < 0.05), followed by Tetric N-Ceram Bulk group. Without BLV application, there was no significant difference among bacterial adhesion values of groups (p > 0.05). CLSM images showed cell viability in groups. Bulk-fill composites showed higher bacterial adhesion than conventional composite and compomer materials. The surface sealant was found to be highly effective in lowering bacterial adhesion, but not so superior in smoothing the surfaces of restorative materials. So, surface sealants could be used on the restorations of patients with high caries risk.Öğe Fracture resistance of endodontically treated premolars restored with CAD-CAM onlays(2022) Ozan, Günce; Eren, Meltem M.; Dikmen, Benin; Yıldız, EsraPurpose: To evaluate the fracture resistance of endodontically treated premolars restored with CAD-CAM onlay restorations. Methods: 60 extracted mandibular first premolars were selected and at first divided into three groups regarding treatment options: MOD onlay with buccal cusp coverage, MOD onlay with buccal cusp coverage + endodontic treatment, MOD onlay with buccal cusp coverage + endodontic treatment + fiber post. Then, all groups were divided into subgroups (n=10) according to the restorative material used: IPS e.max CAD and Lava Ultimate. Each group was submitted to 5,000 thermal cycles, embedded in acrylic resin and secured in a universal testing machine respectively. A compressive load was applied until fracture, at a crosshead speed of 0.5 mm/minute. Statistical significance among each group was analyzed using one-way ANOVA and Bonferroni tests. Results: Statistically, endodontically treated IPS e.max onlays had numerically the lowest average fracture resistance [753.1 (± 224.9) N/mm² ] among all treatment options. IPS e.max onlays treated with fiber posts had significantly higher resistance than that of endodontically treated IPS e.max CAD group (P= 0.013). Endodontically treated teeth restored with Lava Ultimate onlays [1,381.0 (± 471.7) N/mm²] showed significantly higher averages of fracture resistance than IPS e.max CAD onlays. Clinical significance: CAD-CAM composite (resin nanoceramic) onlays resist greater forces compared to ceramic restorations. Fiber posts could improve the fracture resistance of endodontically treated mandibular premolars following the ceramic CAD-CAM onlays.