Investigation of radiotherapeutic effectiveness of Papaya Carica seeds
Yükleniyor...
Dosyalar
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Altınbaş Üniversitesi / Lisansüstü Eğitim Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
For the prevention of cancer, which is a major public health problem, the study of herbal
medicine has been on the rise in recent years. Papaya Carica (PC) is widely used
therapeutically due to its many enzymes, carbohydrates, fatty acids, minerals, vitamins,
phenolic, terpenoids, alkaloids, and sulphur. For this reason, the investigation of the
radiotherapeutic effect of PC seeds was chosen as the subject of this study.
PC seed oils were extracted using two different methods. The first method involved the
extraction of sunflower and PC seed oil (SOPO) obtained by keeping PC seeds in
sunflower oil. The second method involved obtaining PC oil (PPO) solely from pure PC
seeds using an ultrasonic bath. Emulsions were then synthesized using these oils. Varying
amounts of PC oils and emulsions were applied to Human keratinocyte (HaCaT) and
mouse fibroblast (L929) cell lines that were cultured in 96-well plates. The cell lines were
exposed to 6-MV photon beams at doses of 0, 2, 4, and 8 Gy using the Elekta linear
accelerator (Elekta AB, Stockholm, Sweden). After incubating for 72 hours under appropriate conditions, the cells' viability was determined using the MTT assay. Gas
chromatography analyses subsequently identified several phytochemicals with antioxidant
properties in the emulsion forms of SPO and PO. Sunflower oil and PC Seeds Oil
Emulsion (SPOE) as well as Pure PC Seeds Oil Emulsion (POE) contain various important
compounds such as AzA, ALA, L-Arg, Piperine, Diacetic acid, Linoelaidic Acid, DSorbitol, Malic acid, Citric acid and Caffeic acid. The dimensional analyses indicate that
both SPOE and POE were of a Nano size, with SPOE having a determined size of
(293.7±49.42) nm and POE having a determined size of (140.4nm±26.03nm). Our study
discovered that SPOE and POE possess radio protective properties for HaCaT cells after
irradiation with 4Gy. The NOD value for SPOE was (72.24±3.92), whereas the NOD value
for POE was (75.85±2.62). The NOD values indicated that both substances have radio
protective properties since they have a higher NOD value than the control group's NOD
value of (54.00±0.73).
In conclusion, POE demonstrates high radio protective properties attributed to their strong
antioxidant effects. These emulsions can protect healthy tissues before and after
radiotherapy treatment and show promise for use in sunscreen cream formulations.
Açıklama
Anahtar Kelimeler
Papaya Carica Seed Oil, Nano Emulsion, Radio Protective Effect, Phytochemicals, Antioxidant
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Siddiqui, M. T. (2024). Investigation of radiotherapeutic effectiveness of Papaya Carica seeds. (Yayınlanmamış yüksek lisans tezi). Altınbaş Üniversitesi, Lisansüstü Eğitim Enstitüsü, İstanbul.
