Yazar "Abudayyak, Mahmoud" seçeneğine göre listele

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    Chemical composition and in vitro cytotoxicity of Cyclotrichium niveum essential oil against human breast adenocarcinoma (MCF-7) and human lung adenocarcinoma (A549) cells
    (Har Krishan Bhalla and Sons, 2023) Demirbolat, İlker; Karık, Ünal; Nath, Ebru Özdemir; Abudayyak, Mahmoud; Kartal, Murat
    The current study is aimed to investigate the essential oil yields, chemical compositions and in vitro cytotoxic activities of Cyclotrichium niveum (Boiss.) Manden et Scheng. C. niveum is an endemic species of Turkiye, generally used as a spice in local cuisine and traditional medicine. The plant material was collected from Yeşilyurt province of Malatya in August. Hydrodistillation yielded 1.41% (g/g) essential oil. The majority of the essential oil was dominated by oxygenated monoterpenes mainly pulegone (56.83%) and isomenthone (29.06%). Cytotoxicity tests of C. niveum essential oil on MCF-7 (human breast adenocarcinoma cells) and A549 (human lung adenocarcinoma alveolar basal epithelial cells) cell lines represented promising results. IC50 values on MCF-7 and A549 were found to be 31.68 μg/mL and 66.70 μg/mL respectively. In the current work, we have shown for the first time that C. niveum essential oil has cytotoxic properties in the cancer cell lines tested.
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    Chemical composition of different parts of the vitex agnus-castus l. essential oils and their in-vitro cytotoxic activities
    (2023) İnal, Ebrar; Nath, Ebru Ozdemir; Abudayyak, Mahmoud; Ulusoy, Şeyma; İnan, Hatice Akbal; Çicek, Mahmut; Kartal, Murat
    The essential oil (EO) compositions and chemotypes of the important ethnomedicinal plant Vitex agnus-castus L. flowers, leaves, and fruits collected from Turkiye (Balikesir and Istanbul) were identified in the present study. Different parts of the V. agnus-castus EO's in-vitro cytotoxic effects on the MCF-7 (human breast adenocarcinoma) and A549 (human lung carcinoma) human-origin cell lines were anaylzed in the current study. The composition of hydrodistiled EOs extracted from flowers, leaves, and fruits of V. agnus-castus were analyzed by GC-FID/MS. Monoterpene hydrocarbons and oxygenated monoterpene compounds were detected as the predominant component class of the V. agnus-castus. EOs extracted from Balikesir region were defined as the "apinene-1,8-cineole" chemotype, while EOs extracted from Istanbul region were defined as the "sabinene-1,8-cineole" chemotype. Sesquiterpene hydrocarbons constituted more than 20% of the compounds in the EOs extracted from the flowers. To the best of our knowledge, this study is the first to analyze the in-vitro cytotoxic effects of flowers. This study is also the first to show the in-vitro cytotoxic effects of fruit, the most commonly used part of the plant, EO on the MCF-7 cell line. Balikesir region's EOs were observed as more potent-especially the purple flower's IC50 is about 4.68 mu g/mL on the MCF7 cell line- than Istanbul regions, which might be attributed to the higher amount of a-pinene, caryophyllene, and limonene content. Our results indicated that the V. agnus-castus EOs, which contain a-pinene, 1,8-cineole, caryophyllene, and limonene as major components, showed relatively high cytotoxic effects compared to the control groups on the MCF7 and A549 cell lines.
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    Investigation of the Biological Activities of Alcea calvertii
    (Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Abudayyak, Mahmoud; Kanbolat, Seyda; Ergene, Reyyan; Batur, Seyma; Aliyazicioglu, Rezzan
    Herbs are widely used in the treatment of diseases as colds, infections, and cancer. In this work, we evaluate Alcea calvertii, which is a perennial herbaceous plant belonging to the Malvaceae family. It spreads in Anatolia and Mediterranean region and has important uses in terms of ethnobotany. In this study, it was aimed to evaluate the cytotoxic potentials and to investigate the antioxidant activities of methanol, water, chloroform, and ethyl acetate extracts of the aerial parts of Alcea calvertii. For that, the antioxidant activity of Alcea calvertii was determined by four antioxidant power (FRAP), copper reducing antioxidant capacity (CUPRAC) and 2,2-diphenylpicrylhydrazil (DPPH) radical scavenging activity. The cytotoxicity potential of extracts was assessed in the human lung cancer cell line (A549) by MTT assay. It was observed that the highest antioxidant activity was in the methanol extract and the antioxidant activity increased with increasing extract concentration; The TPC values were between 62.5 - 414.6 GAE mu g mL-1, the FRAP values were between 115.7 - 1321.4 mu M Trolox equivalent g-1, CUPRAC values were between 177.1 1321.4 mu M Trolox equivalent g-1, and IC50 values in DPPH caused cytotoxicity in a concentration dependent manner, the IC50 developing new drugs.
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    Mechanisms underlying citrinin-induced toxicity via oxidative stress and apoptosis-mediated by mitochondrial-dependent pathway in SH-SY5Y cells
    (Taylor and Francis Ltd., 2022) Abudayyak, Mahmoud; Karaman, Ecem Fatma; Ozden, Sibel
    Citrinin (CIT) is a mycotoxin produced as a secondary product by the generaAspergillus,Penicillium,Monascus,and other strains. CIT has the potential for contaminating animal feed and human food suchas maize, wheat, rye, barley, oats, rice, cheese, and sake. Although CIT is primarily known as a nephro-toxic mycotoxin, it also affects other organs, including the liver and bone marrow, and its mechanisms oftoxicity have not been clearly elucidated. There is a further lack of studies investigating the potential forCIT-induced neurotoxicity and its mechanisms. In the current study, SH-SY5Y human neuroblastoma cellline was treated with CIT for 24 h to evaluate various toxicological endpoints, such as reactive oxygenspecies (ROS) production and apoptosis induction. Results indicate that CIT has an IC50value of250.90lM and cell proliferation decreased significantly at 50 and 100lM CIT concentrations. These sameconcentrations also caused elevated ROS production ( 34.76%), apoptosis ( 9.43-fold) and calcium ionmobilization ( 36.52%) in the cells. Results show a significant decrease in the mitochondrial membranepotential ( 86.8%). We also found that CIT significantly upregulated the expression of some genesrelated to oxidative stress and apoptosis, while downregulating others. These results suggest that apop-tosis and oxidative stress may be involved in the mechanisms underlying CIT-induced neurotoxicity.