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Öğe Antimicrobial activity of sapindus mukorossi and saponaria officinalis extracts on streptococcus mutans and enterococcus faecalis(Annals of Medical Research, 2021) Dikmen, Benin; Ozan, GünceAim: Studies on the antimicrobial effectiveness of saponins against oral pathogens are conflicting. Therefore, this study aimed to evaluate the antimicrobial activity of saponins from three different extracts of Sapindus mukorossi and Saponaria officinalis plants against Streptococcus mutans and Enterococcus faecalis. Materials and Methods: The fruit of Sapindus mukorossi and the root of Saponaria officinalis materials were sliced into small pieces and macerated with hexane, ethyl acetate (EtOAc) and methanol (MeOH), in this order. The antimicrobial activity of the extracts against Streptococcus mutans and Enterococcus faecalis was determined by the broth microdilution method based on the minimum inhibitory concentrations (MIC) of each extract. Results: Except for the hexane extract of Saponaria Officinalis, all extracts of both plants had an inhibitory activity against the tested microorganisms. Higher MIC values were obtained against Enterococcus faecalis than Streptococcus Mutans. The lowest MIC values for both Sapindus mukorossi and Saponaria officinalis were against Streptococcus mutans (4 mg/ml). Conclusion: Saponins extracted from Sapindus mukorossi and Saponaria officinalis have a potential antibacterial activity against oral pathogens. They may be used as an ingredient in dental agents.Öğe Effect of desensitizers on the microleakage of previously restored Class V resin composite restorations(Taylor & Francis Ltd, 2016) Özsoy, Alev; Eren, Meltem Mert; Gürbüz, Özge; Dikmen, Benin; Çilingir, Aylin; Erdemir, UğurObjectives: The aim of this in vitro study was to evaluate the effect of different desensitizers' application on the microleakage of previously restored Class V composite resin restorations. Materials and methods: Class V cavities were prepared on the buccal surfaces of 40 extracted human third molars. Forty box-shaped cavities were divided into four groups, based on the desensitizers used (n = 10). All teeth were restored with the same bonding agent and composite material. No desensitizer was applied in the control group. In the experimental groups, BisBlock, Gluma and Universal bonding agents were the desensitizers. The desensitizers were applied after completion of composite restorations according to manufacturers' instructions. All specimens were then thermocycled at 5-55 degrees C, with a 10-s dwell time for 500 cycles. The samples were then immersed in 0.5% methylene blue dye for 24 h, sectioned into two equal halves, evaluated for microleakage using a stereomicroscope at 30x magnification and scored on a scale of 0-3. The data were analysed using the Kruskal-Wallis test at the significance level p < 0.05. Results: There were no significant differences in microleakage after desensitizer application (p > 0.05). However, based on the obtained numerical values in our study, while the BisBlock and bonding groups showed lower microleakage at the occlusal margin, BisBlock, Gluma and bonding group showed lower microleakage at the gingival margin compared to the control group. Conclusions: The application of desensitizers as a post-treatment option could be considered an advisable procedure to minimize microleakage.Öğe Effect of different antioxidants on the microtensile bond strength of an adhesive system to sodium hypochlorite-treated dentin(Quintessence Publishing Co Inc, 2015) Dikmen, Benin; Gürbüz, Özge; Özsoy, Alev; Eren, Meltem Mert; Çilingir, Aylin; Yücel, TanerPurpose: To evaluate the effects of different antioxidant treatments on the microtensile bond strength of an adhesive system to sodium hypochlorite (NaOCl)-treated dentin. Materials and Methods: Thirty extracted third molars were sectioned 3 mm below the occlusal surface and divided into six groups according to the antioxidant treatment received: control group: distilled water; NaOCl group: 5.25% NaOCl and distilled water; proanthocyanidin (PA) group: 5.25% NaOCl, 5% PA and distilled water; 1-week storage group: 5.25% NaOCl and storage for 1 week in distilled water; Accel group: 5.25% NaOCl, Accel, and distilled water; noni group: 5.25% NaOCl, noni fruit juice, and distilled water. NaOCl, PA, Accel, noni, and distilled water were administered for 30 s, 10 min, 30 s, 10 min, and 30 s, respectively. A self-etching adhesive system (Single Bond Universal Adhesive) was applied to each specimen and a resin composite (Filtek Z550) was built up to a height of 5 mm on the dentin surface. Each specimen was serially sectioned to obtain sticks with a cross-sectional area of 1 mm(2), and their microtensile bond strength was determined. Statistical differences between groups were analyzed using one-way ANOVA and Tamhane's T2 test. Results: Microtensile bond strength in the NaOCl group was significantly lower than in all other groups. However, there were no significant differences in the bond strength between the groups treated with different antioxidants. Conclusions: NaOCl significantly reduced the microtensile bond strength of the adhesive system. The application of PA, Accel, and noni fruit juice to NaOCl-treated dentin significantly improved the microtensile bond strength.Öğe Effect of surface sealant on the surface roughness of different composites and evaluation of their microhardness(2020) Gürbüz, Özge; Çilingir, Aylin; Dikmen, Benin; Özsoy, Alev; Mert Eren, MeltemPurpose The aim of this study was to evaluate the effect of a surface sealant on the surface roughness of different composites and compare their microhardness values. Materials and Methods Sixty disc-shaped specimens were prepared and assigned to 6 groups (n =10). Groups were prepared as follows; Group 1 (Herculite XRV Ultra), Group 2 (Beautifil Bulk Restorative) and Group 3 (Filtek Bulk Fill Posterior Restorative). Groups 4, 5, and 6 were prepared by applying a surface sealant (BisCover LV) on the specimens in groups 1, 2 and 3. Surface hardness of the discs in group 1, 2, and 3 and surface roughness of the discs in all groups were measured using the Vickers hardness test and a profilometer, respectively. One-way ANOVA was used to test for differences among the groups. Results No significant differences were observed in the microhardness and roughness between the experimental and control groups for each restorative materials. Group 3 showed the highest surface hardness and group 4 showed the lowest surface roughness values. Conclusion Using the BisCover LV resin after the polishing step has no significant effect on the surface roughness. The highest hardness values were obtained for the Filtek Bulk Fill Posterior Restorative after the polishing step. The smoothest surfaces were obtained for all experimental groups using the BisCover LV resin after the polishing step, Herculite XRV Ultra showed lower average roughness values than the other materials.Öğe Fracture resistance of endodontically treated premolars restored with CAD-CAM onlays(2022) Ozan, Günce; Eren, Meltem M.; Dikmen, Benin; Yıldız, EsraPurpose: To evaluate the fracture resistance of endodontically treated premolars restored with CAD-CAM onlay restorations. Methods: 60 extracted mandibular first premolars were selected and at first divided into three groups regarding treatment options: MOD onlay with buccal cusp coverage, MOD onlay with buccal cusp coverage + endodontic treatment, MOD onlay with buccal cusp coverage + endodontic treatment + fiber post. Then, all groups were divided into subgroups (n=10) according to the restorative material used: IPS e.max CAD and Lava Ultimate. Each group was submitted to 5,000 thermal cycles, embedded in acrylic resin and secured in a universal testing machine respectively. A compressive load was applied until fracture, at a crosshead speed of 0.5 mm/minute. Statistical significance among each group was analyzed using one-way ANOVA and Bonferroni tests. Results: Statistically, endodontically treated IPS e.max onlays had numerically the lowest average fracture resistance [753.1 (± 224.9) N/mm² ] among all treatment options. IPS e.max onlays treated with fiber posts had significantly higher resistance than that of endodontically treated IPS e.max CAD group (P= 0.013). Endodontically treated teeth restored with Lava Ultimate onlays [1,381.0 (± 471.7) N/mm²] showed significantly higher averages of fracture resistance than IPS e.max CAD onlays. Clinical significance: CAD-CAM composite (resin nanoceramic) onlays resist greater forces compared to ceramic restorations. Fiber posts could improve the fracture resistance of endodontically treated mandibular premolars following the ceramic CAD-CAM onlays.Öğe Giomer bulk-fill, hibrit bulk-fill ve nano-hibrit kompozitlerin farklı uygulama protokolleri kullanılarak mekanik özelliklerinin karşılaştırılması: In vitro çalışma.(Turkiye Klinikleri. Dishekimligi Bilimleri Dergisi, 2021) Çilingir, Aylin; Dikmen, Benin; Gürbüz, Özge; Özsoy, AlevBu çalışmada, farklı tabaka kalınlıklarında uygulanan düşük ve yüksek viskoziteli bulk-fill giomerler, bir nano-hibrit kompozit ve bir bulk-fill hibrit kompozitin basma dayanımlarıyla bükülme dayanımlarının karşılaştırılması amaçlanmıştır. Gereç ve Yöntemler: Kompozit malzemelerin basma dayanımlarının değerlendirilmesi amacıyla 6 grupta (n=10) toplam 60 adet silindir şeklinde örnek (6x3mm) hazırlanmıştır. Deney grupları aşağıdaki şekildedir: Grup 1 (G1) Tetric N-Ceram (TNC) (2+2+2 mm), Grup 2 (G2) Tetric N-Ceram Bulk-Fill (TBF) (4+2 mm), Grup 3 (G3) Beautifill Bulk-Fill Flowable (BBF) (4+2 mm), Grup 4 (G4) Beautifill Bulk Restorative (BBR) (4+2 mm), Grup 5 (G5) 2 mm BBF+4 mm BBR, Grup 6 (G6) 4 mm BBF+2 mm BBR. Örnekler 1 mm/dk kafa hızında basma dayanımı testine tabi tutulmuştur. Bükülme dayanımı ve bükülme modülüsünün hesaplanabilmesi amacıyla 40 adet (25x2x2 mm) (n=10) kompozit örnek hazırlanmış [TNC (GF1), TBF (GF2), BBR (GF3), BBF (GF4)] ve 3 nokta bükülme testi (0,5 mm/dk) gerçekleştirilmiştir. Elde edilen veriler, tek yönlü ANOVA ve Tamhane T2 testleri kullanılarak değerlendirilmiştir (p0,05). GF4’ün ortalama bükülme dayanımı, GF1, GF2 ve GF3 gruplarından istatistiksel olarak anlamlı derecede yüksek bulunurken, diğer gruplar arasında anlamlı bir fark bulunamamıştır. Sonuç: En yüksek basma ve bükülme dayanımı değerleri, bulk-fill giomerlerle elde edilmiştir. BBR altında uygulandığında BBF kalınlığını 2 mm’den 4 mm’ye çıkarmak, basma dayanımını istatistiksel olarak anlamlı şekilde artırmıştır.Öğe Mechanical properties of bulk-fill versus nanohybrid composites: Effect of layer thickness and application protocols(Universidade Estadual Paulista, Institute of Science and Technology of Sao Jose dos Campos, 2019) Çilingir, Aylin; Özsoy, Alev; Mert-Eren, Meltem; Behram, Özge; Dikmen, Benin; Özcan, MutluObjective: The objective of this study was to evaluate the compressive strength, flexural strength and flexural modulus of high-viscosity, low-viscosity bulk-fill, and conventional nano-hybrid resin composite materials alone and when covered with nano-hybrid resin composite at different incremental thicknesses on the bulk-fill composites. Material and Methods: Specimens (N=60) were fabricated from the following materials or their combinations (n=10 per group): a) conventional nano-hybrid composite Z550 (FK), b) high-viscosity bulk-fill composite (Tetric N Ceram-TBF), c) low-viscosity bulk-fill composite SDR (SDR), d) Sonicfill (SF), e) SDR (2 mm)+FK (2 mm), f) SDR (4 mm)+FK (4 mm). After 24 h water storage, compressive strength was measured in a universal testing machine (1 mm/min). Additional specimens (N=40) (25x2x2 mm3) were made from FK, TBF, SDR and SF in order to determine the flexural strength and the flexural modulus, (n=10) and subjected to three-point bending test (0.5 mm/min). Data were analyzed using one-way ANOVA and Tamhane’s T2 post-hoc tests (p<0.05). Results: The mean compressive strength (MPa) of the nano-hybrid composite (FK) was significantly higher (223.8±41.3) than those of the other groups (123±27-170±24) (p<0.001). SDR (4 mm)+FK (2 mm) showed significantly higher compressive strength than when covered with 4 mm (143±30) or when used alone (146±11) (p<0.05). The mean flexural strength (159±31) and the flexural modulus of FK (34±7) was significantly higher than that of the high-or low-viscosity bulk-fill composites (p<0.001). The mean flexural strength of SF (132±20) was significantly higher compared to TBF (95±25) (p<0.05). Conclusion: Bulk-fill resin composites demonstrated poorer mechanical properties compared to nano-hybrid composite but similar to that of SF. Increasing the thickness of low-viscosity bulk-fill composite (SDR) from 2 to 4 mm underneath the nano-hybrid composite (FK) can improve the mechanical properties of the bulk-fill composites. © 2019, Universidade Estadual Paulista, Institute of Science and Technology of Sao Jose dos Campos. All rights reserved.
