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Naturally occuring nitrate and nitrite in nutrients : determinations in Anatolian honey-bee pollens by capillary electrophoresis
(Altınbaş Üniversitesi, 2021) Kalaycıoğlu, Zeynep; Karadeniz, H. Sena; Erim, F. Bedia
The aim of this work is the simultaneous determination of nitrate and nitrite in a set of bee-pollens of Anatolia using a capillary electrophoretic technique. Separation and determination conditions were 30 mM formic acid/formate buffer at pH 4.0. In earlier steps with pollen samples, only nitrate anion was detected. Consequently, a sample stacking method was applied to increase the detection sensitivity of both anions for the pollen samples. In a stacking operation, the conductivity of separation buffer should be higher than the sample zone. The conductivity of the separation buffer was increased by the addition of 30 mM sodium sulfate. Injection condition was 160 s at 50 mbar. The calibration curves show linear dynamic ranges from 1.5 to 30 μmol/L and 1.5 to 10 μmol/L with correlation coefficients of 0.989 and 0.999 for nitrate and nitrite ions, respectively. All the pollen samples contained appreciable amounts of nitrate (from 2.880 ± 0.055 to 18.72 ± 0.46 mg/kg). Low levels of nitrite were found in all samples (from 0.048 ± 0.008 to 0.301 ± 0.001 mg/kg).