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    Öğe
    Enhancing Radiotherapy Tolerance With Papaya Seed-Derived Nanoemulsions
    (Malden, 2025) Siddiqui, Muhammad Tariq; Ölçeroğlu, Bilge; Gümüş, Zinar Pınar; Şenışık, Ahmet Murat; Barlas, Fırat Barış
    Flavonoid-rich plant materials have gained attention for their potential to reduce radiotherapy side effects. Carica papaya (CP) seeds, known for high flavonoid content, hold promise for therapeutic applications. This study explored the extraction and evaluation of two oils-sunflower oil-based papaya oil (SPO) and pure papaya oil (PPO)-and their nano emulsions (SPOE and PPOE), derived from CP seeds, for radioprotective effects. Chemical analysis using QTOF-MS revealed antioxidants and phytochemicals in the oils and emulsions. Size analysis and zeta potential measurements using dynamic light scattering (DLS) showed particle sizes of 140 ± 26.06 nm for PPOE and 293.7 ± 49.42 nm for SPOE. Post-radiation, both SPOE and PPOE significantly enhanced cell viability, with values of 72.24 ± 3.92 (p ≤ 0.001) and 75.85 ± 2.62 (p ≤ 0.001), respectively. These nanoemulsions show potential as topical agents for reducing radiation-induced tissue damage in radiotherapy. Despite the promising in vitro findings, further in vivo studies are needed to confirm the clinical relevance of these nanoemulsions. Additionally, their incorporation into sunscreen formulations could provide further protection against radiation-induced skin damage, broadening their potential applications.
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    Öğe
    Investigation of radiotherapeutic effectiveness of Papaya Carica seeds
    (Altınbaş Üniversitesi / Lisansüstü Eğitim Enstitüsü, 2024) Siddiqui, Muhammad Tariq; Şenışık, Ahmet Murat
    For the prevention of cancer, which is a major public health problem, the study of herbal medicine has been on the rise in recent years. Papaya Carica (PC) is widely used therapeutically due to its many enzymes, carbohydrates, fatty acids, minerals, vitamins, phenolic, terpenoids, alkaloids, and sulphur. For this reason, the investigation of the radiotherapeutic effect of PC seeds was chosen as the subject of this study. PC seed oils were extracted using two different methods. The first method involved the extraction of sunflower and PC seed oil (SOPO) obtained by keeping PC seeds in sunflower oil. The second method involved obtaining PC oil (PPO) solely from pure PC seeds using an ultrasonic bath. Emulsions were then synthesized using these oils. Varying amounts of PC oils and emulsions were applied to Human keratinocyte (HaCaT) and mouse fibroblast (L929) cell lines that were cultured in 96-well plates. The cell lines were exposed to 6-MV photon beams at doses of 0, 2, 4, and 8 Gy using the Elekta linear accelerator (Elekta AB, Stockholm, Sweden). After incubating for 72 hours under appropriate conditions, the cells' viability was determined using the MTT assay. Gas chromatography analyses subsequently identified several phytochemicals with antioxidant properties in the emulsion forms of SPO and PO. Sunflower oil and PC Seeds Oil Emulsion (SPOE) as well as Pure PC Seeds Oil Emulsion (POE) contain various important compounds such as AzA, ALA, L-Arg, Piperine, Diacetic acid, Linoelaidic Acid, DSorbitol, Malic acid, Citric acid and Caffeic acid. The dimensional analyses indicate that both SPOE and POE were of a Nano size, with SPOE having a determined size of (293.7±49.42) nm and POE having a determined size of (140.4nm±26.03nm). Our study discovered that SPOE and POE possess radio protective properties for HaCaT cells after irradiation with 4Gy. The NOD value for SPOE was (72.24±3.92), whereas the NOD value for POE was (75.85±2.62). The NOD values indicated that both substances have radio protective properties since they have a higher NOD value than the control group's NOD value of (54.00±0.73). In conclusion, POE demonstrates high radio protective properties attributed to their strong antioxidant effects. These emulsions can protect healthy tissues before and after radiotherapy treatment and show promise for use in sunscreen cream formulations.