Mechanisms underlying citrinin-induced toxicity via oxidative stress and apoptosis-mediated by mitochondrial-dependent pathway in SH-SY5Y cells

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dc.contributor.authorAbudayyak, Mahmoud
dc.contributor.authorKaraman, Ecem Fatma
dc.contributor.authorOzden, Sibel
dc.date.accessioned2023-07-21T08:35:28Z
dc.date.available2023-07-21T08:35:28Z
dc.date.issued2022en_US
dc.departmentFakülteler, Eczacılık Meslek Bilimleri Bölümü, Klinik Eczacılık Ana Bilim Dalıen_US
dc.description.abstractCitrinin (CIT) is a mycotoxin produced as a secondary product by the generaAspergillus,Penicillium,Monascus,and other strains. CIT has the potential for contaminating animal feed and human food suchas maize, wheat, rye, barley, oats, rice, cheese, and sake. Although CIT is primarily known as a nephro-toxic mycotoxin, it also affects other organs, including the liver and bone marrow, and its mechanisms oftoxicity have not been clearly elucidated. There is a further lack of studies investigating the potential forCIT-induced neurotoxicity and its mechanisms. In the current study, SH-SY5Y human neuroblastoma cellline was treated with CIT for 24 h to evaluate various toxicological endpoints, such as reactive oxygenspecies (ROS) production and apoptosis induction. Results indicate that CIT has an IC50value of250.90lM and cell proliferation decreased significantly at 50 and 100lM CIT concentrations. These sameconcentrations also caused elevated ROS production ( 34.76%), apoptosis ( 9.43-fold) and calcium ionmobilization ( 36.52%) in the cells. Results show a significant decrease in the mitochondrial membranepotential ( 86.8%). We also found that CIT significantly upregulated the expression of some genesrelated to oxidative stress and apoptosis, while downregulating others. These results suggest that apop-tosis and oxidative stress may be involved in the mechanisms underlying CIT-induced neurotoxicity.en_US
dc.identifier.citationAbudayyak, M., Karaman, E. F., & Ozden, S. (2022). Mechanisms underlying citrinin-induced toxicity via oxidative stress and apoptosis-mediated by mitochondrial-dependent pathway in SH-SY5Y cells. Drug and Chemical Toxicology. 10.1080/01480545.2022.2113095en_US
dc.identifier.issn0148-0545
dc.identifier.scopus2-s2.0-85137773307
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://hdl.handle.net/20.500.12939/3573
dc.indekslendigikaynakScopus
dc.institutionauthorAbudayyak, Mahmoud
dc.language.isoen
dc.publisherTaylor and Francis Ltd.en_US
dc.relation.ispartofDrug and Chemical Toxicology
dc.relation.isversionof10.1080/01480545.2022.2113095en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - İdari Personel ve Öğrencien_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApoptosisen_US
dc.subjectCitrininen_US
dc.subjectNeurotoxicityen_US
dc.subjectReactive Oxygen Speciesen_US
dc.subjectSH-SY5Y Cellsen_US
dc.titleMechanisms underlying citrinin-induced toxicity via oxidative stress and apoptosis-mediated by mitochondrial-dependent pathway in SH-SY5Y cells
dc.typeArticle

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