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Öğe An in vitro assessment of teething gels' effects on human gingival mesenchymal stem cells(2024) Birant, Sinem; İlisulu, Sabiha Ceren; Kılıç, Senem; Tunca, Zeynep; Özcan, Hazal; Akkoç, Tunç; Seymen, FigenBackground: The aim of this study is to examine the cytotoxic effects of dental gels with different contents, which are frequently used during teething, on gingival mesenchymal stem cells (G-MSCs). Method: The teething gels used in this study were Dentinox, Gengigel, Osanite, and Jack and Jill. The human gingival mesenchimal stem cells (hG-MSCs) were incubated with these teething gel solutions (0.1%, 50% and 80% concentrations). Reproductive behavior of G-MSCs was monitored in real time for 72 h using the xCELLigence real-time cell analyzer (RTCA) system. Two-way repeated Anova test and post hoc Bonferroni test were used to evaluate the effect of concentration and dental gel on 0-hour and 72-hour viability. Significance was evaluated at p < 0.05 level. Results: Teething gels prepared at 50% concentration are added to the G-MSC culture, the "cell index" value of G-MSCs to which Dentinox brand gel is added is significantly lower than all other groups (p = 0.05). There is a statistically significant difference between the concentrations in terms of cell index values at the 72nd hour compared to the 0th hour (p = 0.001). Conclusions: The local anesthetic dental gels used in children have a more negative effect on cell viability as concentration increases.Öğe Analyzing effects on anterior open bite in twins by PLS-SEM and sobel test(2024) Birant, Sinem; Veznikli, Mert; Kasımoğlu, Yelda; Koruyucu, Mine; Evren, Atıf Ahmet; Seymen, FigenObjective: This study aimed to assess the different pathways between predictor factors such as zygosity, atypical swallowing, mouth breathing, breastfeeding and bottle feeding related to anterior open bite (AOB) in twins. Methods: The study was conducted in monozygotic (MZ) and dizygotic (DZ) twin children aged 3-15 years. AOB, atypical swallowing, mouth breathing, feeding type, duration of bottle use, and mouth opening status during sleep were recorded during oral examination. Partial least squares structural equation model (PLS-SEM) and sobel tests were performed to assess the total and indirect effects among the variables on AOB. Results: A total of 404 children (29.2% MZ;70.8% DZ) participated in this study. The effect of zygosity on mouth breathing in the PLS-SEM model was statistically significant. Conversely, it was determined that mouth breathing effected that atypical swallowing (p = 0.001). Atypical swallowing triggered AOB (p = 0.001). The atypical swallowing has a mediation effect between AOB and mouth breathing (p = 0.020). Mouth breathing causes atypical swallowing and therefore indirectly increases the likelihood of AOB. While breastfeeding decreases AOB incidence (p = 0.023), bottle feeding increases AOB incidence (p = 0.046). The sobel tests show that the fully mediator variable feature of mouth breathing is statistically significant in the negative relation between zygosity and atypical swallowing. Conclusion: The PLS-SEM model showed that mouth breathing triggers atypical swallowing and atypical swallowing triggers AOB. As a result of this chain of relationships, an indirect effect of zygosity on AOB was observed. According to sobel tests, zygosity has an indirect effect on atypical swallowing through mouth breathing, while mouth breathing has a positive indirect effect on AOB through atypical swallowing. Clinical relevance: This study identified the relationships between different factors and the presence of AOB. The findings of this study demonstrate in detail the relationships between AOB and zygosity, atypical swallowing, mouth breathing, breastfeeding and bottle feeding. Brestfeeding has a reducing effect on the frequency of AOB. Among the nutritional forms, breastfeeding ensures the proper development of the stomatognathic system by working the oro-facial muscles.Öğe Clinical assessment of deleterious oral habits and dental caries–periodontal parameters among Turkish twins(Association for Dental Sciences of the Republic of China, 2023) İlisulu, Sabiha Ceren; Birant, Sinem; Kasimoglu, Yelda; Koruyucu, Mine; Tuna İnce, Elif Bahar; Seymen, FigenAbstract Background/purpose: Twin studies are crucial to assess the relative contribution of genetic and environmental factors. This study was conducted to evaluate association between deleterious oral habits and dental caries–periodontal parameters among Turkish twins. Materials and methods: The study comprised 143 pairs of dizygotic (DZ) twins and 59 pairs of monozygotic (MZ) twins aged 3–15 years. Twins were examined for dental caries, plaque index, gingival index, bleeding on probing and deleterious oral habits. Mann Whitney U test was used to examine the data. Results: The MZ twin pairs consisted of 60 male and 58 female twin pairs, whereas the DZ twin pairs consisted of 144 male and 142 female. The mean age of the twins was 9.63 ± 3.0 in MZ twins and 9.47 ± 3.2 in DZ twins. The mean DMFS value of MZ twins with bruxism is higher than those of MZ twins without bruxism (P = 0.001). The mean DMFS value of DZ twins with pacifier sucking is lower than those without (P = 0.007). A statistically significant difference was found between MZ twins with and without nail biting in terms of bleeding on probing and dmfs values (P = 0.035; P = 0.012). The mean values of the plaque index increased due to the mouth breathing in DZ twins (P = 0.024). Regarding the bleeding on probing, there was a statistically significant difference between MZ twins with and without atypical swallowing (P = 0.016). Conclusion: These findings suggest that dental caries–periodontal parameters are similarly affected by deleterious oral habits in MZ and DZ twins.Öğe Cytotoxic efects of diferent detergent containing children’s toothpastes on human gingival epithelial cells(BMC Oral Health, 2022) Birant, Sinem; Duran, Yazgül; Akkoç, TunçBackground: This study aimed to evaluate possible cytotoxic efects to gingival epithelial cells exposed to children toothpastes containing diferent detergent. Methods: Tissues required for the isolation of human gingival epithelial cells were obtained by biopsy during the extraction of the impacted third molar tooth. Toothpaste solutions of diferent concentrations were prepared from fve diferent children’s toothpastes with diferent detergent contents. Isolated gingival epithelial cells were stimulated with experimental groups consisting of toothpaste solutions (Colgate, Sensodyne, Splat, Nenedent, Perlodent) at diferent concentrations and a control group consisting of complete Dulbecco’s modifed eagle medium. After the experiments, cell viability was evaluated using fow cytometry. 2 Way ANOVA was used to see the interaction efect of the main efects of toothpaste solution and concentration factors. Pairwise comparisons were made by Tukey post hoc tests. In the study, the signifcance level was taken as 0.05. Results: As a result of the analysis, it was seen that the toothpaste solution and concentration factors and the interactions of these 2 factors were efective on the viable, early apoptotic, late apoptotic and necrotic cell rates. The statistically highest live cell ratios were detected in Splat’s toothpaste solutions (90.14% at 0.4% concentration) after the control group (90.82%) and the group with the lowest viability values was determined in Colgate group (75.74% at 0.4% concentration)Öğe Examination of the effect of treatment of severe early childhood caries and fluoride varnish applications on salivary oxidative stress biomarkers and antioxidants(2024) Birant, Sinem; İlisulu, Sabiha Ceren; Özcan, Hazal; Yanar, KarolinBackground: Saliva contains a variety of biochemical compounds, including antioxidants, and serves as the body's first line of defense against oxidative stress caused by free radicals. The aim of this study was to investigate the effects of dental treatments on salivary oxidative stress biomarkers in children aged 3-5 years with severe early childhood caries (S-ECC) compared to children without caries. Method: This study was conducted on 20 children aged 3-5 years with severe early childhood caries (S-ECC) and 20 children without caries. Salivary oxidative stress biomarkers and antioxidants were measured after the initial examination (T0), after the end of restorative treatments (T1), and after fluoride varnish applications (T2). Post hoc Bonferroni test was used to compare normally distributed parameters between T0-T1-T2 times. Pearson correlation analysis was used to examine the relationships between parameters that conform to normal distribution. The Mann-Whitney U test was used to compare the parameters in the control and experimental groups. Significance was evaluated at the p < 0.05 level. Results: The mean dmft of the participants in the study group was 8.86 ± 14.5. Advanced oxidation protein products (AOPP), dityrosine (DT), kynurenine (KYN), advanced glycation end products (AGE), lipid hydroperoxides (LHP) and malondialdehyde (MDA) values decrease after the treatment of dental caries and protective fluoride varnish applications, while an increases in total thiol (TSH) and Cu/Zn-superoxide dismutase (Cu/Zn-SOD) values were observed after protective varnish applications compared to pre-treatment values. Antioxidant parameters at time T2 in the study group were statistically significantly higher than in the control group (p < 0.05). In the study group, there was no correlation between TSH and oxidative stress mediators in terms of changes at time T1 post-treatment compared to the pre-treatment period, while an inverse moderate relationship was found with AGE and LHP in terms of changes at time T2 post-treatment (p < 0.05). Conclusions: An increase in salivary antioxidants was detected after dental restorations were completed and protective fluoride varnish application, while a decrease in oxidative stress markers was detected. Clinical relevance: Fluoride varnish applications applied in the study group may further reduce the oral microbiome load and cause salivary oxidative stress markers to be significantly lower than in the control group.Öğe İkizlerde Genetik Ve Çevresel Faktörlerin Dental Bulgulara Etkisi(2018) Seymen, Figen; Karacan, İlker; Tuna İnce, Elif Bahar; Gençay, Koray; Nursen Topcuoğlu, Emine; Koruyucu, Mine; Birant, SinemDiş çürüklerinin ortaya çıkmasında genetiğin rolü önemlidir, hastalığın %30-60 arasında kalıtsal yatkınlık ile oluştuğu bildirilmektedir. Bu amaçla, ikizlerde ağız diş sağlığını ilgilendiren klinik bulgular detaylı bir muayene ile değerlendirilerek kardeşler arasında farklılıkların olup olmadığının belirlenmesi, genetik ve metagenomik analizler ile de ağız diş sağlığını ilgilendiren klinik parametreler ile çürük genetiği üzerine aday genlerin ve mikroorganizmaların etkilerinin incelenmesi hedeflenmiştir. Çalışmaya, İstanbul Üniversitesi Diş Hekimliği Fakültesi Pedodonti Anabilim Dalı?na ilk muayene için başvuran 3-15 yaş aralığındaki (ort. yaş 9,64±3,28) 404 birey (143 çift yumurta ikizi, 59 tek yumurta ikizi) dahil edilmiştir. Aileden detaylı anamnez alınarak çocukların doğum haftası, doğum boyu ve kilosu kaydedilmiştir. Klinik muayene ile ağız kokusu varlığı, çürük veya dolgulu dişlerin oranı, periodontal bulgular, dişlerdeki eroziv/hipomineralize/hipokalsifiye lezyonlar, ve dental anomaliler belirlenmiştir. Maloklüzyona neden olabilecek kötü ağız alışkanlıkları (parmak emme, ağızdan solunum, biberon emzik kullanımı, tırnak yeme, atipik yutkunma), eklem problemleri ve bruksizm hakkında veliye sorular sorulmuştur. Radyografik muayene ile çocukların büyüme gelişim yaşı ve dişlere ait anomaliler değerlendirilmiştir. Çalışma modelleri üzerinden dişlerin büyüklükleri, ark boyu ve diş boyu ölçümleri yapılmıştır. Çalışma kapsamında bireylerin steril tek kullanımlık tükürük kaplarına tükürmeleri sağlanmıştır. Alınan tükürük örneklerinden DNA izolasyonu yapıldıktan sonra diş çürüğüne neden olduğu literatürlerde belirtilen genlerin markırları PCR?da incelenerek mutasyonlar tespit edilmiştir. Bu analizin sonucuna göre çürük yapıcı genlerin metagenomik incelenmesi ile rastgele seçilmiş bireylerde oral profil değerlendirilmiştir. Çalışmada elde edilen bulgular değerlendirilirken, istatistiksel analizler için SPSS22 programı kullanılmıştır. Çalışma verileri değerlendirilirken; Oneway, ANOVA, Kruskal Wallis, post hoc Tukey HSD testi, Mann Whithney U testi, Bonferroni testi, Friedman Testi, Wilcoxon işaret testi ve Fisher?s exact test kullanılmıştır. Sonuç olarak rs3796704 (ENAM) ve rs3828054 (TUFT1) SNP?lerinin çürük ile ilişkili olduğu belirlenmiştir. Gruplarda diş çürüğü olan, olmayan ya da kardeşlerin sadece birisinde diş çürüğü bulunan ikizlerde ise hem ağırlıklandırılmamış hem de ağırlıklandırılmış UniFrac metrik hesabına göre gruplar arasında mikrobiyom benzerliği açısından anlamlı farklılıklar bulunmamıştır.Öğe Investigation of the self-reported attitude of dentists towards preventive applications(2023) Koruyucu, Mine; Özcan, Hazal; Birant, Sinem; İlisulu, Sabiha Ceren; Bekiroglu, Nural; Seymen, FigenThe purpose of this study was to determine the approaches of dentists to the use of fluoride applications as preventive treatment options. The study was a questionnaire based cross-sectional survey divided into two sections and the participating dental practitioners were asked to answer a questionnaire about: (i) demographic data and (ii) dentists' attitudes to prevention. Data entry and analyses were performed using SPSS statistical software. Data were analyzed using frequency counts, percentages, and Chi-square test. The sample (N=481), who volunteered to participate, consisted of 332 general dentists (69%) and 149 specialist dentists (31%). 380 (79%) dentists applied preventive applications. 452 dentists (94%) considered that fluoride was effective in preventing caries. 235 dentists (48.9%) thought that fluoride had side effects. Significantly more female dentists thought that fluoride had a caries preventive effect than male dentists (p=0.005) and they applied preventive treatments more frequently than male dentists (p=0.01). All age groups believed that fluoride was useful to prevent caries, and almost all the dentists in the 18-25 age range believed in a caries preventive effect of fluoride (98.6%) (p=0.0001). Dentists in 18-25 age range used preventive applications for their patients more than other age groups (p=0.019). Fewer dentists aged 26-35 than dentists in the other age groups (p=0.037) thought that the fluoride preparations had dental and/or medical side effects. Non-specialist dentists gave more priority to preventive applications than specialist dentists (p=0.0001). The approach of dentists to the use of preventive practices will change if they keep up-todate with information about preventive practices through ongoing training.Öğe Parents' perspective towards dental radiography for children(Association for Dental Sciences of the Republic of China, 2023) Birant, Sinem; İlisulu, Sabiha Ceren; Özcan, HazalBackground/purpose: Radiographs provide critical information on developmental and eruption problems, detection of interface caries, pulpal and periapical pathologies in clinical examination. This study aimed to evaluate parents' knowledge, attitudes and behaviors towards dental radiographs. Materials and methods: A cross-sectional study was conducted on the parents of 396 children who visited to the Pediatric Dentistry clinics. All participants were asked to answer a total of 26 questions. A questionnaire was developed to assess participants' attitudue, behavior and knowledge toward pediatric dental radiographies and provided information on their demographic characteristics. The number of positive attitudes and correct answers was scored based on items asked to the participants. In order to analyze the effect of demographic data on the obtained scores, a multivariate linear regression model was created. The significance level was accepted as 0.05 in all analyzes. Results: Children's dental radiography experience affects parents' attitude score towards dental radiographs (t = 4.375, P < 0.001). Parents' level of knowledge about dental radiography has a positive effect on their attitudes (t = 5.510, P = 0.005). The education level of the parents and the frequency of visits to the dentist have a significant effect on the knowledge level of the parents about dental radiography (P < 0.05). When the correlation between knowledge and attitude scores is analyzed, it is observed that there is a statistically significant, weak and positive correlation between the two variables (r = 0.34, P < 0.001). Conclusion: In conclusion, parents who did not had dental radiographs before have a more negative attitude towards dental radiographs than those who have had dental radiographs.Öğe Path analysis of the relationships between the eruption time of the first primary teeth and various factors in twins(MDPI, 2023) Birant, Sinem; Veznikli, Mert; Kasımoğlu, Yelda; Koruyucu, Mine; Evren, Atıf Ahmet; Seymen, FigenThe timing of primary tooth eruption is critical for children’s health planning and the diagnosis of specific growth disorders. The purpose of this study is to assess the relationship between twin pairs’ birth weight, gestational age, and gender, which are indicators of prenatal factors; breast-feeding duration, which is an indicator of postnatal factors; type of delivery, which is an indicator of maternal as well as genetic factors; and age of the primary tooth. Twin children aged from 3 to 15 years who applied to the clinic for the first dental examination constituted the sample group. In this twin study, 59 monozygotic (MZ) twin pairs and 143 dizygotic (DZ) twin pairs were included. Genetic (MZ vs. DZ), maternal (type of delivery, gestational age), perinatal (birth weight, gender), and postnatal (duration of breastfeeding) information was obtained, and effects on the children’s Eruption Timing of the First Primary Tooth (ETFPT) were examined. Statistical analysis was performed using the consistent partial least squares structural equation model (robust PLSc) technique. As birth weight increased, the age at first eruption became younger, but this change was different between MZ and DZ twins (p < 0.05). While the age at first tooth eruption was older in identical twins who were breastfed for the first 6 months, this increase was not observed in DZ twins. The mean of ETFPT was calculated as 7.31 months in MZ twins and 6.75 months in DZ twins. The effect of breastfeeding and birth weight on ETFPT may differ according to zygosity in twins. MZ twins may tend to take longer to experience the eruption of their first primary teeth.
