Cytotoxic efects of diferent detergent containing children’s toothpastes on human gingival epithelial cells
Yükleniyor...
Dosyalar
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
BMC Oral Health
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background: This study aimed to evaluate possible cytotoxic efects to gingival epithelial cells exposed to children toothpastes containing diferent detergent. Methods: Tissues required for the isolation of human gingival epithelial cells were obtained by biopsy during the extraction of the impacted third molar tooth. Toothpaste solutions of diferent concentrations were prepared from fve diferent children’s toothpastes with diferent detergent contents. Isolated gingival epithelial cells were stimulated with experimental groups consisting of toothpaste solutions (Colgate, Sensodyne, Splat, Nenedent, Perlodent) at diferent concentrations and a control group consisting of complete Dulbecco’s modifed eagle medium. After the experiments, cell viability was evaluated using fow cytometry. 2 Way ANOVA was used to see the interaction efect of the main efects of toothpaste solution and concentration factors. Pairwise comparisons were made by Tukey post hoc tests. In the study, the signifcance level was taken as 0.05. Results: As a result of the analysis, it was seen that the toothpaste solution and concentration factors and the interactions of these 2 factors were efective on the viable, early apoptotic, late apoptotic and necrotic cell rates. The statistically highest live cell ratios were detected in Splat’s toothpaste solutions (90.14% at 0.4% concentration) after the control group (90.82%) and the group with the lowest viability values was determined in Colgate group (75.74% at 0.4% concentration)
Açıklama
Anahtar Kelimeler
Toothpaste, Stem Cell, Annexin V, Detergent, SLS
Kaynak
BMC Oral Health
WoS Q Değeri
Q1
Scopus Q Değeri
Q2
Cilt
22
Sayı
1
Künye
Birant, S., Duran, Y., Akkoc, T., & Seymen, F. (2022). Cytotoxic effects of different detergent containing children's toothpastes on human gingival epithelial cells. BMC Oral Health, 22(1), 1-16.
