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Öğe Chemical components of thymbra spicata subsp. spicata l. essential oil and its in vitro physiological effects on human origin cell lines(2023) Nath, Ebru Özdemir; Gündoğan, Gül I.; Kartal, MuratThymbra spicata subsp. spicata L. is a member of the Lamiaceae family, which is used in Türkiye against various health issues among other uses. The main components of T. spicata essential oil (TE) were identified as carvacrol (52.3%) and p-cymene (21.1%) using GC-FID and GC/MS. The in vitro effects of TE on Miapaca-2 and HUVEC cell lines were reported for the first time. The initial results showed that TE applied to Miapaca-2 cell lines at concentrations of ≥62.18 g/mL for 48 hours could reduce cell growth and induce apoptosis. The application of relatively high concentrations of TE (≥82.91 g/mL) significantly (P<0.001) suppressed cell growth. Administration of relatively high TE concentrations (≥82.91 g/mL) significantly (P<0.001) suppressed cell growth. Concentrations of TE lower than 20.73 g/mL did not affect the viability of the HUVEC cell line in 48 hours. The IC50 value for Miapaca-2 cells was 62.18 µg/mL, and HUVEC cells’ IC50 value was 263.97 µg/mL for 48h. The number of apoptotic cells in Miapaca-2 (55±5%) and HUVEC (38.33±6%) were significantly higher (P<0.001). Significantly lower migration rates (P<0.001) were seen for Miapaca-2 (52±5%) and HUVEC cell lines (64±1.67%).Öğe Chemical composition and in vitro cytotoxicity of Cyclotrichium niveum essential oil against human breast adenocarcinoma (MCF-7) and human lung adenocarcinoma (A549) cells(Har Krishan Bhalla and Sons, 2023) Demirbolat, İlker; Karık, Ünal; Nath, Ebru Özdemir; Abudayyak, Mahmoud; Kartal, MuratThe current study is aimed to investigate the essential oil yields, chemical compositions and in vitro cytotoxic activities of Cyclotrichium niveum (Boiss.) Manden et Scheng. C. niveum is an endemic species of Turkiye, generally used as a spice in local cuisine and traditional medicine. The plant material was collected from Yeşilyurt province of Malatya in August. Hydrodistillation yielded 1.41% (g/g) essential oil. The majority of the essential oil was dominated by oxygenated monoterpenes mainly pulegone (56.83%) and isomenthone (29.06%). Cytotoxicity tests of C. niveum essential oil on MCF-7 (human breast adenocarcinoma cells) and A549 (human lung adenocarcinoma alveolar basal epithelial cells) cell lines represented promising results. IC50 values on MCF-7 and A549 were found to be 31.68 μg/mL and 66.70 μg/mL respectively. In the current work, we have shown for the first time that C. niveum essential oil has cytotoxic properties in the cancer cell lines tested.Öğe Chemical composition and In Vitro Cytotoxicity of endemic Thymus brachychilus jalas against human breast adenocarcinoma (MCF-7, HTB-22), human lung adenocarcinoma (A549, CRM-CCL-185), and human glioblastoma cells (U-118 MG, HTB-15)(2024) Nath, Ebru Ozdemir; Abudayyak, Mahmou; Demirbolat, Ilker; Sönmez, Sefa; Kartal, MuratObjective: Thymus have 341 species in the world and 46 species in Turkiye, 19 of them are endemic. Thymus species are used for stomachache, cold, shortness of breath, cough, bronchitis, diabetes in Aladalar (Nide). In this study, essential oil (EO) analysis of Thymus brachychilus, liquid chromatography/mass spectrometry (LC/MS) analysis of ethanol extract, and its effects on the cell lines mentioned in the title were investigated for the first time. Methods: The endemic Thymus brachychilus Jalas was collected from Aladalar Mountains, from 3,290 meters. Voucher specimens were prepared after the plant species were identified and kept at the herbarium (HERA 1029). 100 g of dried and powdered aerial part of the plant material was distilled for three hours, yielding 0.42 mL of volatile oil using a Clevenger-style apparatus. The 3.28 g ethanol extract was obtained from 100 g plant. An Agilent GC-FID/MS system was used to analyze EO samples. Phenolic compounds of the extract were analyzed using LC-HRMS. MTT assay was used to evaluate the cytotoxicity of EO and ethanol extract. Results: Thymol (48.11%), p-cymene (12.92%), carvacrol (11.14%) were the major components of the EO. For the extract, the IC50 values were calculated to be between 1.64-15.76 mu g/mL, while the values of IC50 were calculated between 68.94-101.2 mu g/ mL for the essential oil. Conclusion: Both the ethanol extract and the EO caused cell death in the tested cell lines. However, the extract appeared to be more effective compared to the essential oil. Besides that, a difference between the cell types could be seen, where the U118 MG neuronal cells appeared to be the most sensitive cell line.Öğe Chemical composition of different parts of the vitex agnus-castus l. essential oils and their in-vitro cytotoxic activities(2023) İnal, Ebrar; Nath, Ebru Ozdemir; Abudayyak, Mahmoud; Ulusoy, Şeyma; İnan, Hatice Akbal; Çicek, Mahmut; Kartal, MuratThe essential oil (EO) compositions and chemotypes of the important ethnomedicinal plant Vitex agnus-castus L. flowers, leaves, and fruits collected from Turkiye (Balikesir and Istanbul) were identified in the present study. Different parts of the V. agnus-castus EO's in-vitro cytotoxic effects on the MCF-7 (human breast adenocarcinoma) and A549 (human lung carcinoma) human-origin cell lines were anaylzed in the current study. The composition of hydrodistiled EOs extracted from flowers, leaves, and fruits of V. agnus-castus were analyzed by GC-FID/MS. Monoterpene hydrocarbons and oxygenated monoterpene compounds were detected as the predominant component class of the V. agnus-castus. EOs extracted from Balikesir region were defined as the "apinene-1,8-cineole" chemotype, while EOs extracted from Istanbul region were defined as the "sabinene-1,8-cineole" chemotype. Sesquiterpene hydrocarbons constituted more than 20% of the compounds in the EOs extracted from the flowers. To the best of our knowledge, this study is the first to analyze the in-vitro cytotoxic effects of flowers. This study is also the first to show the in-vitro cytotoxic effects of fruit, the most commonly used part of the plant, EO on the MCF-7 cell line. Balikesir region's EOs were observed as more potent-especially the purple flower's IC50 is about 4.68 mu g/mL on the MCF7 cell line- than Istanbul regions, which might be attributed to the higher amount of a-pinene, caryophyllene, and limonene content. Our results indicated that the V. agnus-castus EOs, which contain a-pinene, 1,8-cineole, caryophyllene, and limonene as major components, showed relatively high cytotoxic effects compared to the control groups on the MCF7 and A549 cell lines.Öğe Development of phytotherapeutic nanoformulation containing Gypsophila eriocalyx and its evaluation as a candidate formulation for osteoporosis treatment on human bone marrow stem cells(2024) Kaymak, Sibel; Kurtur, Ozan Barış; Gök, Bahar; Budama-Kılınç, Yasemin; Keçel-Gündüz, Serda; Nath, Ebru Özdemir; Kartal, MuratIntroduction: Osteoporosis, one of the common bone diseases, manifests itself as a decrease in bone mass. Recently, the use of medicinal plants in the search for effective and low-toxicity therapeutics for the prevention or treatment of osteoporosis has become a trending topic. Objective: In this study, we aim to prepare a controlled drug carrier system loaded with Gypsophila eriocalyx to determine its potential for anti-osteoporosis applications. Methods: Gypsophila eriocalyx extract (GEE) was prepared, and components were determined. The molecular interactions of the components with Cathepsin K (CatK), which is used as a target in drug development against osteoporosis, were revealed by in silico molecular docking and MD methods. ADMET profiles were also examined. GEE-loaded chitosan nanoparticles (CNPs) were synthesized. The nanoparticles' morphology, encapsulation efficiency, loading capacity, release profile, average size, polydispersity index, and zeta potentials were determined. The cytotoxic effects of GEE and GEE-loaded CNPs on the L929 and osteogenic proliferation profiles on human bone marrow stem cells (hBMC) were examined. Results: The MD analysis revealed no breaks or atomic changes in the dynamic system, and the docking analysis confirmed the continued interaction of identical residues. It was determined that the GEE-loaded CNP formulation was produced successfully, had no toxic effect on the L929, and had an osteogenic proliferation effect on hBMC. Conclusion: In line with the in vitro and in silico results obtained, it was evaluated that GEE-loaded CNPs can be used as a controlled drug release system as a candidate formulation with phytotherapeutic properties for osteoporosis treatment.q1.
