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  • Küçük Resim
    Öğe
    Biomolecular changes and subsequent time-dependent recovery in hippocampal tissue after experimental mild traumatic brain injury
    (2021) Garip Ustaoğlu, Şebnem; Mohamed H. M.A, Ali; Rakib, Fazle; Blezer, Erwin L. A.; Heijningen, Caroline L.Van; Dijkhuizen, Rick M.; Severcan, Feride
    Traumatic brain injury (TBI) is the main cause of disability and mortality in individuals under the age of 45 years. Elucidation of the molecular and structural alterations in brain tissue due to TBI is crucial to understand secondary and long-term efects after traumatic brain injury, and to develop and apply the correct therapies. In the current study, the molecular efects of TBI were investigated in rat brain at 24 h and 1 month after the injury to determine acute and chronic efects, respectively by Fourier transform infrared imaging. This study reports the time-dependent contextual and structural efects of TBI on hippocampal brain tissue. A mild form of TBI was induced in 11-week old male Sprague Dawley rats by weight drop. Band area and intensity ratios, band frequency and bandwidth values of specifc spectral bands showed that TBI causes signifcant structural and contextual global changes including decrease in carbonyl content, unsaturated lipid content, lipid acyl chain length, membrane lipid order, total protein content, lipid/protein ratio, besides increase in membrane fuidity with an altered protein secondary structure and metabolic activity in hippocampus 24 h after injury. However, improvement and/or recovery efects in these parameters were observed at one month after TBI.
  • Küçük Resim
    Öğe
    Fourier transform infrared imaging—A novel approach to monitor bio molecular changes in subacute mild traumatic brain injury
    (MDPI, 2021) Rakib, Fazle; Al-Saad, Khalid; Garip Ustaoğlu, Şebnem; Ullah, Ehsan; Mall, Raghvendra; Thompson, Richard; Abdelalim, Essam M.; Ahmed, Tariq; Severcan, Feride; Ali, Mohamed H. M.
    Traumatic brain injury (TBI) can be defined as a disorder in the function of the brain after a bump, blow, or jolt to the head, or penetrating head injury. Mild traumatic brain injury (mTBI) can cause devastating effects, such as the initiation of long-term neurodegeneration in brain tissue. In the current study, the effects of mTBI were investigated on rat brain regions; cortex (Co) and corpus callosum (CC) after 24 h (subacute trauma) by Fourier transform infrared (FTIR) imaging and immunohistochemistry (IHC). IHC studies showed the formation of amyloid-β (Aβ) plaques in the cortex brain region of mTBI rats. Moreover, staining of myelin basic protein presented the shearing of axons in CC region in the same group of animals. According to FTIR imaging results, total protein and lipid content significantly decreased in both Co and CC regions in mTBI group compared to the control. Due to this significant decrease in both lipid and protein content, remarkable consistency in lipid/protein band ratio in mTBI and control group, was observed. Significant decrease in methyl content and a significant increase in olefinic content were observed in Co and CC regions of mTBI rat brain tissues. Classification amongst distinguishable groups was performed using principal component analysis (PCA) and hierarchical clustering (HCA). This study established the prospective of FTIR imaging for assessing biochemical changes due to mTBI with high sensitivity, precision and high-resolution.