Fourier transform infrared imaging—A novel approach to monitor bio molecular changes in subacute mild traumatic brain injury
Yükleniyor...
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
MDPI
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Traumatic brain injury (TBI) can be defined as a disorder in the function of the brain after
a bump, blow, or jolt to the head, or penetrating head injury. Mild traumatic brain injury (mTBI)
can cause devastating effects, such as the initiation of long-term neurodegeneration in brain tissue.
In the current study, the effects of mTBI were investigated on rat brain regions; cortex (Co) and
corpus callosum (CC) after 24 h (subacute trauma) by Fourier transform infrared (FTIR) imaging and
immunohistochemistry (IHC). IHC studies showed the formation of amyloid-β (Aβ) plaques in the
cortex brain region of mTBI rats. Moreover, staining of myelin basic protein presented the shearing
of axons in CC region in the same group of animals. According to FTIR imaging results, total protein
and lipid content significantly decreased in both Co and CC regions in mTBI group compared to the
control. Due to this significant decrease in both lipid and protein content, remarkable consistency in
lipid/protein band ratio in mTBI and control group, was observed. Significant decrease in methyl
content and a significant increase in olefinic content were observed in Co and CC regions of mTBI
rat brain tissues. Classification amongst distinguishable groups was performed using principal
component analysis (PCA) and hierarchical clustering (HCA). This study established the prospective
of FTIR imaging for assessing biochemical changes due to mTBI with high sensitivity, precision and
high-resolution.
Açıklama
Anahtar Kelimeler
Fourier Transform Infrared, Traumatic brain injury, Biochemical Analysis and Immunohistochemistry
Kaynak
Brain Sciences
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
11
Sayı
7
Künye
Rakib, F., Al-Saad, K., Ustaoglu, S. G., Ullah, E., Mall, R., Thompson, R., ... & Ali, M. H. (2021). Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury. Brain Sciences, 11(7), 918.
