Immobilized lipase on micro-porous biosilica for enzymatic transesterification of algal oil

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dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorAkbulut, Aydın
dc.contributor.authorÖzalp, Veli Cengiz
dc.contributor.authorArıca, M. Yakup
dc.date.accessioned2021-05-15T12:40:32Z
dc.date.available2021-05-15T12:40:32Z
dc.date.issued2015
dc.departmentTıp Fakültesi, Biyokimya Anabilim Dalıen_US
dc.descriptionOzalp, Veli Cengiz/0000-0002-7659-5990
dc.description.abstractEnzymatic transesterification reactions for biodiesel production require harsh conditions, which require methods of enzyme stability enhancements. In this study, we present covalently immobilized lipase on the biosilica-polymer composite as a viable method to obtain enzymes with enhanced stability in such harsh conditions. The fresh water microalgae Scenedesmus quadricauda was cultivated in a batch photo-bioreactor with CO2 aeration, and urea was supplied as nitrogen source (0.075 g L-1). Under optimized conditions, the amount of extracted oil was around 29.6%. Finally, the algal oil was utilized for production of biodiesel via enzymatic transesterification reaction which were performed in n-hexane using the free and immobilized lipase preparations. Fatty acid methyl ester (FAME) components were determined using gas chromatography-mass spectrophotometry (GC-MS). The conversion of algal oil to biodiesel was found to be 85.7% and 96.4%, with the free and immobilized enzyme, respectively. The immobilized lipase was highly stable and only 17% of activity was lost after 6 cycles repeated uses. (c) 2015 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipHacettepe UniversityHacettepe University [013D06604002]; Hacettepe UniversityHacettepe University [013D06604002]en_US
dc.description.sponsorshipFinancial support from Hacettepe University (Research Grant/013D06604002) for this research is gratefully acknowledged.en_US
dc.identifier.doi10.1016/j.cherd.2014.12.011
dc.identifier.endpage21en_US
dc.identifier.issn0263-8762
dc.identifier.issn1744-3563
dc.identifier.scopus2-s2.0-84926016419
dc.identifier.scopusqualityQ2
dc.identifier.startpage12en_US
dc.identifier.urihttps://doi.org/10.1016/j.cherd.2014.12.011
dc.identifier.urihttps://hdl.handle.net/20.500.12939/606
dc.identifier.volume95en_US
dc.identifier.wosWOS:000351966600002
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorÖzalp, Veli Cengiz
dc.language.isoen
dc.publisherInst Chemical Engineersen_US
dc.relation.ispartofChemical Engineering Research & Design
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiosilicaen_US
dc.subjectLipaseen_US
dc.subjectImmobilized Lipaseen_US
dc.subjectAlgal Oilen_US
dc.subjectTransesterificationen_US
dc.subjectBiodieselen_US
dc.titleImmobilized lipase on micro-porous biosilica for enzymatic transesterification of algal oil
dc.typeArticle

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