Heterologous expression, biochemical characterisation and computational analysis of Bacteroides fragilis enolase
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Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Bacteriodes fragilis is an anaerobic bacterium found in the human intestinal flora. In this study, BfEno was targeted with a structure-based drug design approach because inhibition of this enzyme may prevent both the aerobic and anaerobic pathways due to its role in the glycolytic pathway. First, the gene encoding BfEno was cloned, expressed and the protein produced over 95% purity. The Km and Vmax values of BfEno were determined as 314.9 µM and 256.2 µmol/min.mg, respectively. Drug-like chemicals were retrieved from the ZINC database for high-throughput virtual screening analyses. As a result of screening study, the ZINC91441604 has been proposed to bind to the active site of the enzyme and remain stable. The same compound exhibited weak binding to the human enolases than the bacterial enolase. Hence, ZINC91441604 may be proposed as a novel candidate for further in vitro and in vivo drug analysis towards the treatment of B. fragilis infections.
Açıklama
Anahtar Kelimeler
Bacteriodes Fragilis, Enolase, Antibiotic Resistance, Structure-Based Drug Design, Enzyme Kinetic, In Silico Studies
Kaynak
Computational Biology and Chemistry
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
Sayı
Künye
Ugurel, E., Kocer, S., Sariyer, E., Mutlu, O., Inci, T. G., Ugurel, O. M., & Turgut-Balik, D. (2022). Heterologous expression, biochemical characterisation and computational analysis of bacteroides fragilis enolase. Computational Biology and Chemistry.