Evaluation of Bacteriophage ?11 host recognition protein and its host-binding peptides for diagnosing/targeting of Saphylococcus aureus infections

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dc.contributor.authorDokuz, Senanur
dc.contributor.authorTaşdurmazlı, Semra
dc.contributor.authorAcar, Tayfun
dc.contributor.authorDuran, Gizem Nur
dc.contributor.authorÖzdemir, Çilem
dc.contributor.authorÖzbey, Utku
dc.contributor.authorÖzbil, Mehmet
dc.contributor.authorKaradayı, Şükriye
dc.contributor.authorBayrak, Ömer Faruk
dc.contributor.authorDerman, Serap
dc.contributor.authorChen, John Yu-Shen
dc.contributor.authorÖzbek, Tülin
dc.date.accessioned2024-06-24T11:48:47Z
dc.date.available2024-06-24T11:48:47Z
dc.date.issued2024en_US
dc.departmentMeslek Yüksekokulları, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Laboratuvar Teknikleri Programıen_US
dc.description.abstractEvaluating the potential of using both synthetic and biological products as targeting agents for the diagnosis, imaging, and treatment of infections due to particularly antibiotic-resistant pathogens is important for controlling infections. We examined the interaction between Gp45, a receptor-binding protein of the ϕ11 lysogenic phage, and its host S. aureus, a common cause of nosocomial infections. Using molecular dynamics and docking simulations, we identified the peptides that bind to S. aureus wall teichoic acids via Gp45. We compared the binding affinity of Gp45 and the two highest-scoring peptide sequences (P1 and P3) and their scrambled forms using microscopy, spectroscopy, and ELISA. Our results revealed that rGp45 (recombinant Gp45) and chemically synthesized P1 had a higher binding affinity for S. aureus compared with all other peptides, with the exception of E. coli. Furthermore, rGp45 had a capture efficiency of over 86%; P1 had a capture efficiency of over 64%. Overall, our findings suggest that receptor-binding proteins such as rGp45, which provide a critical initiation of the phage life cycle for host adsorption, might play an important role in the diagnosis, imaging, and targeting of bacterial infections. Studying such proteins could accordingly enable the development of effective strategies for controlling infections.en_US
dc.identifier.citationDokuz, S., Taşdurmazlı, S., Acar, T., Duran, G. N., Özdemir, Ç., Özbey, U., Özbil, M., Karadayı, Ş., Bayrak, Ö. F., Derman, S., Chen, J. Y., Özbek, T. (2024). Evaluation of Bacteriophage ϕ11 host recognition protein and its host-binding peptides for diagnosing/targeting of Saphylococcus aureus infections. International Journal of Antimicrobial Agents, 64(2). 10.1016/j.ijantimicag.2024.107230en_US
dc.identifier.issn0924-8579
dc.identifier.issn1872-7913
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85198101123
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://hdl.handle.net/20.500.12939/4727
dc.identifier.volume64en_US
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKaradayı, Şükriye
dc.language.isoen
dc.relation.ispartofInternational Journal of Antimicrobial Agents
dc.relation.isversionof10.1016/j.ijantimicag.2024.107230en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectReceptor binding proteinen_US
dc.subjectS. aureusen_US
dc.subjectAntimicrobial resistanceen_US
dc.subjectTargeting peptideen_US
dc.subjectϕ 11 phageen_US
dc.titleEvaluation of Bacteriophage ?11 host recognition protein and its host-binding peptides for diagnosing/targeting of Saphylococcus aureus infections
dc.typeArticle

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