THE USE OF ACTIVIN IN OVARIAN FOLLICLE CULTURE SYSTEMS
[ X ]
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Nova Science Publishers, Inc.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The cancer treatment during puberty and early adulthood might have negative effects on reproductive system in women, causing premature menopause, premature ovarian failure and infertility. In order to protect germ cells from these undesirable effects without any functional loss, different experimental approaches have been developed. One of these approaches is ovarian follicle culture system which includes in vitro growth of ovarian follicles obtained from the patients before being exposed to the detrimental effects of cancer treatment. In vitro growth of the follicles starts from primordial stage and gives rise to an ovulatory follicle with a competent oocyte within a culture system supplemented with folliculogenesis activators. Activin is one of these follicle activators supporting the growth and survival of the follicles. As a member of the transforming growth factor beta (TGF-β) superfamily, activin is known to be expressed in granulosa cells and oocytes. The main role of activin is stimulation of follicle stimulating hormone (FSH) secretion from anterior pituitary and induction of follicle growth enabling granulosa cell proliferation and antral cavity formation. Exogenous activin has been considered to be one of the key components of the in vitro follicle culture. The effects of the activin during in vitro follicle growth have been evaluated through both morphological and molecular biological criteria such as oocyte and follicle diameter, oocyte integrity, expressions of proliferation markers in granulosa cells and expressions of mitogenactivated protein kinase (MAPK) and Akt signaling members in the follicles, meiotic competence of oocytes and hormone synthesis. Since, one of the most important effects of activin on granulosa cells is induction of FSH receptor expression, activin is mostly used in combination with FSH during the culture. The current literature has mainly focused on the differential effects of activin on in vitro grown follicles at different developmental stages in the presence and absence of other activators such as FSH. This review covers the key literature on the structural and functional effects of the use of activin during in vitro growth of ovarian follicles. © 2020 by Nova Science Publishers, Inc. All rights reserved.
Açıklama
Anahtar Kelimeler
Activin, Follicle culture, Oocyte maturation, Ovary
Kaynak
Advances in Medicine and Biology: Volume 160
WoS Q Değeri
Scopus Q Değeri
Cilt
160
