An investigation on the effects of SIRT5 modulators on SIRT5 and cytochrome C protein expressions in K562 chronic myeloid leukemia cell line
Yükleniyor...
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
University of Ankara
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: SIRT5 is a mitochondrial protein that removes acetyl, malonyl and succinyl groups from lysine moieties in target proteins and interacts with cytochrome c and causes its deacetylation. There is no study on the effects of SIRT5 in K562 chronic myeloid leukemia cells. Resveratrol and Suramin are known to play a role in modulating the deacetylase and desuccinylase activities of SIRT5. It has been reported that Resveratrol induces apoptosis of K562 cells but effects of Suramin on the apoptosis of K562 cells are largely unknown. In this study, it was aimed to elucidate the effects of SIRT5 modulators Resveratrol and Suramin on proliferation and apoptosis of K562 cells and on SIRT5 and cytochrome c protein, a known target of SIRT5. Material and Method: K562 chronic myeloid leukemia cells were treated with increasing concentrations of suramin and resveratrol, cell proliferation was determined by MTT assay and BrdU incorporation. Apoptosis was determined with Annexin V staining by Flow cytometry. Western Blot analysis was performed to determine the effect of resveratrol and suramin on SIRT5 and Cytochrome c protein expression levels. Result and Discussion: Our results showed that suramin did not affect SIRT5 and cytochrome c protein expressions significantly and resveratrol decreased SIRT5 and increased cytochrome c expression. Suramin did not cause any changes on the apoptosis of K562 cells. Resveratrol decreased cell proliferation and induced apoptosis of K562 cells in accordance with the literature. The SIRT5-lowering effect of Resveratrol may have mediated its apoptotic effects.
Açıklama
Anahtar Kelimeler
Apoptosis, K562, Resveratrol, SIRT5, Suramin
Kaynak
Ankara Universitesi Eczacilik Fakultesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
46
Sayı
3
Künye
Özkan, T., Koç, A., Karabay, A. Z., Hekmatshoar, Y., Sunguroğlu, A. (2022). An investigation on the effects of SIRT5 modulators on SIRT5 and cytochrome C protein expressions in K562 chronic myeloid leukemia cell line/K562 kronik miyeloid lösemi hücre hattında SIRT5 modülatörlerinin SIRT5 ve sitokrom C protein ekspresyonları üzerine etkilerinin araştırılması. Journal of Faculty of Pharmacy of Ankara University, 46(3), 805-816.
