An investigation on the effects of SIRT5 modulators on SIRT5 and cytochrome C protein expressions in K562 chronic myeloid leukemia cell line

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dc.contributor.authorÖzkan, Tülin
dc.contributor.authorKoç, Aslı
dc.contributor.authorKarabay, Arzu Zeynep
dc.contributor.authorHekmatshoar, Yalda
dc.contributor.authorSunguroğlu, Asuman
dc.date.accessioned2023-03-23T09:51:09Z
dc.date.available2023-03-23T09:51:09Z
dc.date.issued2022en_US
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri, Tıbbi Biyoloji Ana Bilim Dalıen_US
dc.description.abstractObjective: SIRT5 is a mitochondrial protein that removes acetyl, malonyl and succinyl groups from lysine moieties in target proteins and interacts with cytochrome c and causes its deacetylation. There is no study on the effects of SIRT5 in K562 chronic myeloid leukemia cells. Resveratrol and Suramin are known to play a role in modulating the deacetylase and desuccinylase activities of SIRT5. It has been reported that Resveratrol induces apoptosis of K562 cells but effects of Suramin on the apoptosis of K562 cells are largely unknown. In this study, it was aimed to elucidate the effects of SIRT5 modulators Resveratrol and Suramin on proliferation and apoptosis of K562 cells and on SIRT5 and cytochrome c protein, a known target of SIRT5. Material and Method: K562 chronic myeloid leukemia cells were treated with increasing concentrations of suramin and resveratrol, cell proliferation was determined by MTT assay and BrdU incorporation. Apoptosis was determined with Annexin V staining by Flow cytometry. Western Blot analysis was performed to determine the effect of resveratrol and suramin on SIRT5 and Cytochrome c protein expression levels. Result and Discussion: Our results showed that suramin did not affect SIRT5 and cytochrome c protein expressions significantly and resveratrol decreased SIRT5 and increased cytochrome c expression. Suramin did not cause any changes on the apoptosis of K562 cells. Resveratrol decreased cell proliferation and induced apoptosis of K562 cells in accordance with the literature. The SIRT5-lowering effect of Resveratrol may have mediated its apoptotic effects.en_US
dc.identifier.citationÖzkan, T., Koç, A., Karabay, A. Z., Hekmatshoar, Y., Sunguroğlu, A. (2022). An investigation on the effects of SIRT5 modulators on SIRT5 and cytochrome C protein expressions in K562 chronic myeloid leukemia cell line/K562 kronik miyeloid lösemi hücre hattında SIRT5 modülatörlerinin SIRT5 ve sitokrom C protein ekspresyonları üzerine etkilerinin araştırılması. Journal of Faculty of Pharmacy of Ankara University, 46(3), 805-816.en_US
dc.identifier.endpage816en_US
dc.identifier.issn1015-3918
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85149910840
dc.identifier.scopusqualityQ3
dc.identifier.startpage805en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12939/3454
dc.identifier.volume46en_US
dc.indekslendigikaynakScopus
dc.institutionauthorHekmatshoar, Yalda
dc.language.isoen
dc.publisherUniversity of Ankaraen_US
dc.relation.ispartofAnkara Universitesi Eczacilik Fakultesi Dergisi
dc.relation.isversionof10.33483/jfpau.1081893en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectApoptosisen_US
dc.subjectK562en_US
dc.subjectResveratrolen_US
dc.subjectSIRT5en_US
dc.subjectSuraminen_US
dc.titleAn investigation on the effects of SIRT5 modulators on SIRT5 and cytochrome C protein expressions in K562 chronic myeloid leukemia cell line
dc.title.alternativeK562 kronik miyeloid lösemi hücre hattında SIRT5 modülatörlerinin SIRT5 ve sitokrom C protein ekspresyonları üzerine etkilerinin araştırılması
dc.typeArticle

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AN-INVESTIGATION-ON-THE-EFFECTS-OF-SIRT5-MODULATORS-ON-SIRT5-AND-CYTOCHROME-C-PROTEIN-EXPRESSIONS-IN-K562-CHRONIC-MYELOID-LEUKEMIA-CELL-LINEAnkara-Universitesi-Eczacilik-Fakultesi-Dergisi.pdf
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