Lysozyme specific aptamer immobilized MCM-41 silicate for single-step purification and quartz crystal microbalance (QCM)-based determination of lysozyme from chicken egg white

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dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorÖzalp, V. Cengiz
dc.contributor.authorYılmaz, Meltem
dc.contributor.authorGüler, Ulku
dc.contributor.authorSalih, Bekir
dc.contributor.authorArıca, M. Yakup
dc.date.accessioned2021-05-15T12:37:52Z
dc.date.available2021-05-15T12:37:52Z
dc.date.issued2015
dc.departmentTıp Fakültesi, Biyokimya Anabilim Dalıen_US
dc.descriptionOzalp, Veli Cengiz/0000-0002-7659-5990; guler, ulku/0000-0001-6914-9362; YILMAZ, MELTEM/0000-0001-8070-8487
dc.description.abstractIn the present study, MCM-41 silica particles were functionalized with 3-aminopropyltriethoxysilane (APTES) and characterized by FTIR, SEM, TEM and, Brunauer Emmett Teller (BET) analysis. A lysozyme specific aptamer was then immobilized onto amine functionalized MCM-41 particles via glutaraldehyde coupling, which were used for adsorption and purification of lysozyme. The effect of various parameters including pH, adsorbent dosage and lysozyme concentration on the lysozyme specific aptamer immobilized silica particles was evaluated. Efficiency of aptamer-silica particles in the purification of lysozyme from diluted chicken egg white was also realized. The optimal adsorption condition was in phosphate buffer (50 mmol L-1, pH 7.0) and the lysozyme adsorption capacity was 36.8 mg g(-1) polymer. Increasing the initial lysozyme concentration and temperature had a positive effect on the binding capacity, whereas increasing the ionic strength resulted in the opposite effect. The recovery of adsorbed lysozyme by elution with glycine buffer (0.2 mol L-1, pH 2.0) was about 93%. Additionally, proteins with different isoelectric points (e.g. lysozyme, albumin, cytochrome c and hemoglobin) were used as model proteins to investigate the selectivity of the lysozyme binding aptamer. The lysozyme aptamer was used in the purification of lysozyme from diluted chicken egg white, which was verified by a single SDS-PAGE band. The reusability studies showed that, about 87% of the initial adsorption capacity of the aptamer immobilized particles could be maintained after 20 cycles of purification. Finally, the specific interaction between lysozyme specific aptamer sequences and lysozyme was investigated by quartz crystal microbalance (QCM) biosensor for determination of lysozyme in the egg samples. (C) 2015 Elsevier Inc. All rights reserved.en_US
dc.identifier.doi10.1016/j.micromeso.2015.01.009
dc.identifier.endpage104en_US
dc.identifier.issn1387-1811
dc.identifier.issn1873-3093
dc.identifier.scopus2-s2.0-84922349607
dc.identifier.scopusqualityQ1
dc.identifier.startpage95en_US
dc.identifier.urihttps://doi.org/10.1016/j.micromeso.2015.01.009
dc.identifier.urihttps://hdl.handle.net/20.500.12939/598
dc.identifier.volume207en_US
dc.identifier.wosWOS:000350518600014
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorÖzalp, V. Cengiz
dc.language.isoen
dc.publisherElsevieren_US
dc.relation.ispartofMicroporous and Mesoporous Materials
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSilicaen_US
dc.subjectAptameren_US
dc.subjectAdsorptionen_US
dc.subjectLysozymeen_US
dc.subjectBiosensoren_US
dc.titleLysozyme specific aptamer immobilized MCM-41 silicate for single-step purification and quartz crystal microbalance (QCM)-based determination of lysozyme from chicken egg white
dc.typeArticle

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