An in vitro assessment of ionizing radiation impact on the efficacy of radiotherapy for breast cancer

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dc.contributor.authorGirgin, Merve
dc.contributor.authorAkat, Ayberk
dc.contributor.authorAkgül, Büşra
dc.contributor.authorNalbant, Nilgül
dc.contributor.authorKaraçetin, Didem
dc.contributor.authorAbamor, Emrah Şefik
dc.contributor.authorUğurel, Osman Mutluhan
dc.contributor.authorTurgut-Balık, Dilek
dc.date.accessioned2024-10-04T09:08:10Z
dc.date.available2024-10-04T09:08:10Z
dc.date.issued2024en_US
dc.departmentFakülteler, Mühendislik ve Doğa Bilimleri Fakültesi, Yazılım Mühendisliği Bölümüen_US
dc.descriptionFunding agency : Yildiz Technical University.
dc.description.abstractObjectives Ionizing radiation is still one of the most effective treatment options for various cancers. It is possible to reduce the side effects of this effective treatment method and increase the chance of success by elucidating the responses it creates at the molecular level in the cell. This study aims to investigate of the molecular effects of therapeutic ionizing radiation on breast cancer, which is the most prevalent cancer type. Methods MDA-MB-231 and MCF7 cell lines were irradiated with 4 and 8 Gy ionizing radiation and monitored for up to 7 days. RNA was collected at 48 and 96 h, when cellular molecular mechanisms became most evident, and quantitative expression levels of microRNAs (miR-208a, miR-124, miR-145), for which cancer-radiation associations have been determined from existing literature and databases, were evaluated. Results Exposure to ionizing radiation resulted in a dose-dependent reduction in cell viability in both MCF7 and MDA-MB-231 breast cancer cell lines. Furthermore, microRNA expression analysis revealed notable changes at all levels. The research demonstrates that miR-208a, miR-145, and miR-124 are crucial in the biological response to ionizing radiation. Conclusions Therapeutic ionizing radiation profoundly affects cell viability and microRNA expression in breast cancer cell lines, showing dose and time-dependent effects. The observed microRNA expression patterns suggest potential biomarkers for radiation response and therapeutic targets to improve radiotherapy efficacy. Further in vivo validation and exploration of these microRNAs' roles in modulating cellular response to ionizing radiation are needed.en_US
dc.identifier.citationGirgin, M., Akat, A., Akgül, B., Nalbant, N., Karaçetin, D., Abamor, E. S., Uğurel, O. M., Turgut-Balık, D. (2024). An in vitro assessment of ionizing radiation impact on the efficacy of radiotherapy for breast cancer. Turkish Journal of Biochemistry. 10.1515/tjb-2024-0172en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.scopus2-s2.0-85204898045
dc.identifier.scopusqualityN/A
dc.identifier.urihttps://hdl.handle.net/20.500.12939/4932
dc.identifier.wosWOS:001315383200001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorUğurel, Osman Mutluhan
dc.language.isoen
dc.relation.ispartofTurkish Journal of Biochemistry-Türk Biyokimya Dergisi
dc.relation.isversionof10.1515/tjb-2024-0172en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectRadiotherapyen_US
dc.subjectIonizing radiationen_US
dc.subjectBreast canceren_US
dc.subjectMiRNAsen_US
dc.subjectMiRNA expressionen_US
dc.titleAn in vitro assessment of ionizing radiation impact on the efficacy of radiotherapy for breast cancer
dc.typeArticle

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