UHPLC-DAD method development and validation for simultaneous quantification of 7 phenolic compounds in dietary supplements of sage capsules

Basit Öğe Kaydı
dc.contributor.authorGözet, Tuba
dc.contributor.authorAlkhidhri, Athar
dc.date.accessioned2025-08-14T14:42:17Z
dc.date.available2025-08-14T14:42:17Z
dc.date.issued2025
dc.departmentFakülteler, Eczacılık Fakültesi
dc.description.abstractThe content of the dietary supplements of capsules generated from extracts of medicinal plants in terms of bioactive compounds may not be known. The aim of this study is to do method development and validation for quantification of the phenolic compounds (chlorogenic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid luteolin-7-o-glucoside, and rosmarinic acid) in capsules of dietary sage (Salvia officinalis) supplements by ultra-high-performance liquid chromatography with diode array detector (UHPLC-DAD) to facilitate the quality control. Mobile phase gradient method is optimized in reverse UHPLC mode using aqueous acetonitrile as the mobile and C18 (50*2.1 mm, 1.8 µm) as the stationary phase. Quantitation was done by signal specific integration using diode array detector (DAD). Total run time is 18 minutes. Separation of the phenolic compounds were at 35 °C by employing gradient elution of water: trifluoroacetic acid (100:0.1, v/v) and acetonitrile: water: trifluoroacetic acid (95:5:0.1, v/v/v) as mobile phase A and B, respectively at a flow rate of 0.5 ml/min. The method showed good linearity with correlation coefficient (r2) that varied between 0.9993 and 0.9997. Limit of detection (LOD) and limit of quantification (LOQ) values were 1.97-18.77 and 6.58-62.56 mg/ml, respectively. Recovery (%) were between 90% and 114% for capsule matrix for all compounds at all levels except for ferulic acid and luteolin-7-o-glucoside. The method is suitable for application in quality control laboratories because of the short run time and simultaneous analysis of phenolic compounds in dietary supplements of sage capsules.
dc.description.sponsorshipFunding agency : Scientific Research Office Grant number : PB2019-ECZ2
dc.identifier.citationGozet, T., & Alkhidhri, A. (2024). UHPLC-DAD method development and validation for simultaneous quantification of 7 phenolic compounds in dietary supplements of sage capsulesa. Journal of Research in Pharmacy, 29(4), 1732-1745.
dc.identifier.doi10.12991/jrespharm.1734720
dc.identifier.endpage1745
dc.identifier.issn2630-6344
dc.identifier.issue4
dc.identifier.scopus2-s2.0-105011763152
dc.identifier.scopusqualityQ3
dc.identifier.startpage1732
dc.identifier.urihttps://hdl.handle.net/20.500.12939/5853
dc.identifier.volume29
dc.identifier.wosWOS:001545457100001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakScopus
dc.indekslendigikaynakWeb of Science
dc.institutionauthorGözet, Tuba
dc.institutionauthorAlkhidhri, Athar
dc.language.isoen
dc.publisherMarmara University
dc.relation.ispartofJournal of Research in Pharmacy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectdietary supplements
dc.subjectmedicinal plants
dc.subjectmethod development and validation
dc.subjectPhenolic compounds
dc.subjectsage
dc.subjectsalvia officinalis
dc.subjectUHPLC
dc.titleUHPLC-DAD method development and validation for simultaneous quantification of 7 phenolic compounds in dietary supplements of sage capsules
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Tam Metin / Full Text
Boyut:
1.34 MB
Biçim:
Adobe Portable Document Format
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.17 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
Eczacılık Fakültesi Yayın Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu