Selection of Aptamers for Metabolite Sensing and Construction of Optical Nanosensors

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dc.authoridMayer, Gunter/0000-0003-3010-4049
dc.authoridOlsen, Lars Folke/0000-0001-7391-2340
dc.authoridLong, Yi/0000-0003-4651-3365
dc.authoridErsoy, Franziska/0000-0001-6800-1825
dc.authoridozalp, cengiz/0000-0002-7659-5990
dc.contributor.authorLong, Yi
dc.contributor.authorPfeiffer, Franziska
dc.contributor.authorMayer, Gunter
dc.contributor.authorSchroder, Tine Daa
dc.contributor.authorOzalp, Veli Cengiz
dc.contributor.authorOlsen, Lars Folke
dc.coverage.doi10.1007/978-1-4939-3197-2
dc.date.accessioned2025-02-06T17:58:25Z
dc.date.available2025-02-06T17:58:25Z
dc.date.issued2016
dc.departmentAltınbaş Üniversitesien_US
dc.description.abstractOptical nanosensors are based on particles with diameters from 20 to 200 nm containing sensory elements. The latter are comprised of one or more signaling molecules and one or more references, which allow measurements to be ratiometric and hence independent on the amount of sensor. The signaling molecules may range from simple ion-binding fluorophores, e.g., pH-sensitive dyes, to complex biochemical assays. Aptamers are ideal for use in nanosensors because they are relatively easy to modify chemically and hence to transform into signaling molecules, and their binding affinities may be fine-tuned to a desired measuring range in the selection process. Here we first describe the selection of metabolite binding aptamers, how they are transformed into signaling molecules using a molecular beacon construct and then how they are inserted into nanoparticles. Finally, we briefly describe how the sensors are calibrated before inserted into cells to measure metabolite concentration in real time. As examples we present aptamers binding to key metabolites in cells: ATP and fructose 1, 6-bisphosphate (FBP).en_US
dc.description.sponsorshipEU FP7 Marie Curie program; Lundbeck Foundationen_US
dc.description.sponsorshipThis research was supported by EU FP7 Marie Curie program to the Initial Training Network ISOLATE. Tine Daa Schroder was supported by the Lundbeck Foundation grant to the Nanomedicine Research Center for Cancer Stem Cell-Targeting Therapeutics (NanoCAN).en_US
dc.identifier.doi10.1007/978-1-4939-3197-2_1
dc.identifier.endpage19en_US
dc.identifier.isbn978-1-4939-3197-2
dc.identifier.isbn978-1-4939-3196-5
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.pmid26552812
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage3en_US
dc.identifier.urihttps://doi.org/10.1007/978-1-4939-3197-2_1
dc.identifier.urihttps://hdl.handle.net/20.500.12939/5240
dc.identifier.volume1380en_US
dc.identifier.wosWOS:000685783400002
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherHumana Press Incen_US
dc.relation.ispartofNucleic Acid Aptamers: Selection, Characterization, and Applicationen_US
dc.relation.publicationcategoryKitap Bölümü - Uluslararasıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKA_WOS_20250206
dc.subjectNanosensoren_US
dc.subjectAptamersen_US
dc.subjectSelectionen_US
dc.subject6-Bisphosphateen_US
dc.subjectFructose 1en_US
dc.subjectATPen_US
dc.subjectCalibrationen_US
dc.titleSelection of Aptamers for Metabolite Sensing and Construction of Optical Nanosensorsen_US
dc.typeBook Parten_US

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