Changes in secondary structure of protein in skeletal muscle due to high-carbohydrate of high-fat diets

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dc.contributor.authorÖzer, Nazlı Ezer
dc.contributor.authorMollaoğlu, Ayça Doğan
dc.date.accessioned2024-12-27T09:22:24Z
dc.date.available2024-12-27T09:22:24Z
dc.date.issued2024en_US
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri, Fizyoloji Ana Bilim Dalıen_US
dc.description.abstractObjective: Obesity, which arises from changes in lifestyle and feeding habits, poses a threat to human health. One essential contributor to the increase in obesity rates is the popularity of high-calorie diets. This study aims to investigate high-fat (HFD) and high-carbohydrate (HCD) diet-induced molecular changes in protein secondary structure in longissimus dorsi skeletal muscle tissues of female inbred C57BL/6J mice by utilizing Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy. Materials and Methods: Mice were fed a control diet, HCD, or HFD for 24 weeks. Their skeletal muscle tissues were collected, and their spectra were recorded using a Bruker Invenio S ATR-FTIR spectrometer in the 4000-400 cm-1 region. Results: The protein secondary structure profiles of the HCD group demonstrated a significant rise in antiparallel beta-sheet and beta-turn and a decline in parallel beta-sheets, together with the insignificant increase in aggregated beta-sheets and a decrease in alpha-helix. The impact of an HFD on protein conformation is less pronounced than HCD. The HFD diet led to an increase in antiparallel beta-sheets and a decrease in parallel beta sheets. Although insignificant, an increase was observed in beta-turn and alpha-helix. Conclusion: These results propose the appearance of protein aggregation and/or formation of protein- protein intermolecular interaction in skeletal muscle tissues of female inbred C57BL/6J mice. Collectively, these data suggest that both high-calorie diets impair secondary structures of protein in skeletal muscle that may affect its metabolic function.en_US
dc.identifier.citationÖzer, N. E., Mollaoğlu, A. D. (2024). Changes in secondary structure of protein in skeletal muscle due to high-carbohydrate of high-fat diets. Meandros Medical and Dental Journal, 25(3), 233-243. 10.69601/meandrosmdj.1537978en_US
dc.identifier.endpage243en_US
dc.identifier.issn2149-9063
dc.identifier.issue3en_US
dc.identifier.startpage233en_US
dc.identifier.trdizinid1267786
dc.identifier.urihttps://hdl.handle.net/20.500.12939/5124
dc.identifier.volume25en_US
dc.identifier.wosWOS:001378426300001
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorMollaoğlu, Ayça Doğan
dc.language.isoen
dc.relation.ispartofMeandros Medical and Dental Journal
dc.relation.isversionof10.69601/meandrosmdj.1537978en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.tubitakTUBITAK-3501-SBAG-118S484
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectObesityen_US
dc.subjectHigh-carbohydrate dieten_US
dc.subjectHigh-fat dieten_US
dc.subjectProtein secondary structureen_US
dc.subjectFTIRen_US
dc.subjectSpectroscopyen_US
dc.titleChanges in secondary structure of protein in skeletal muscle due to high-carbohydrate of high-fat diets
dc.typeArticle

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