Induction of humoral and cell-mediated immunity in mice by chitosan-curdlan composite nanoparticles administered intranasally and subcutaneously

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dc.contributor.authorSessevmez, Melike
dc.contributor.authorSinani, Genada
dc.contributor.authorOkyar, Alper
dc.contributor.authorAlpar, Hazire Oya
dc.contributor.authorCevher, Erdal
dc.date.accessioned2023-07-21T08:33:21Z
dc.date.available2023-07-21T08:33:21Z
dc.date.issued2023en_US
dc.departmentFakülteler, Eczacılık Teknolojisi Bilimleri Bölümü, Farmasötik Teknoloji Ana Bilim Dalıen_US
dc.description.abstractInfectious diseases seriously threaten human life. The need for novel delivery systems and adjuvants suitable to be used in clinic for antigens remains a challenge. Among various attempts that have been made to provide optimum immune responses for protein antigens with poor immunogenicity, polymeric nanoparticles offer great opportunities. In this study, carboxymethyl curdlan (CMC) as a polyanionic polymer and chitosan chloride (CC) and N-trimethyl chitosan (TMC) as polycationic polymers were synthesised in-house and composite nanoparticles (CC-CMC-BSA and TMC-CMC-BSA) loaded with bovine serum albumin (BSA) as a model antigen were prepared via polyelectrolyte complexation and reported here for the first time. Nanoparticles with an average size ranging from 153 nm to 615 nm, positive surface charge (from +24 mV to +55 mV) and encapsulation efficiency as high as 90% were obtained depending on the concentration of polymers in the formulation. The nanoparticles showed good physical stability for three months and low toxicity in Calu-3 and A549 cells. Furthermore, SDS integrity of antigen was maintained. In vivo studies in mice indicated that nasal and subcutaneous administration of composite nanoparticles could provide long-term humoral and cellular immunity as determined by serum antibody titres (IgG, IgG1, IgG2a) and cytokine (IL-2, IL-4, IL-6, IL-10 and IFN-γ) levels. Elevated sIgA levels after nasal administration of the nanoparticles showed that mucosal immunity was successfully stimulated. These findings suggest that chitosan-curdlan composite nanoparticles show optimum properties to be used as nanovaccine for nasal immunisation of protein antigens.en_US
dc.identifier.citationSessevmez, M., Sinani, G., Okyar, A., Alpar, H. O., & Cevher, E. (2023). Induction of humoral and cell-mediated immunity in mice by chitosan-curdlan composite nanoparticles administered intranasally and subcutaneously. Journal of Drug Delivery Science and Technology, 86, 104704.en_US
dc.identifier.issn1773-2247
dc.identifier.scopus2-s2.0-85163847224
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://hdl.handle.net/20.500.12939/3571
dc.identifier.volume86en_US
dc.identifier.wosWOS:001053998300001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorSinani, Genada
dc.institutionauthorAlpar, Hazire Oya
dc.language.isoen
dc.publisherEditions de Santeen_US
dc.relation.ispartofJournal of Drug Delivery Science and Technology
dc.relation.isversionof10.1016/j.jddst.2023.104704en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChitosanen_US
dc.subjectCurdlanen_US
dc.subjectImmune responseen_US
dc.subjectNanoparticleen_US
dc.subjectNasalen_US
dc.subjectVaccineen_US
dc.titleInduction of humoral and cell-mediated immunity in mice by chitosan-curdlan composite nanoparticles administered intranasally and subcutaneously
dc.typeArticle

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