Yazar "Hernandez, Luiza I." seçeneğine göre listele

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    Nanocapsules in biomedicine promises and challenges
    (Scrivener Publishing Llc, 2015) Hernandez, Frank J.; Kavruk, Murat; Hernandez, Luiza I.; Özalp, Veli Cengiz
    With the recent advances in nanotechnology, in the past decade, the ways of doing biomedicine have been reshaped considerably. Nanometer-size structures (nanoparticles, nanowires, nanocapsules, etc.) are used in biosensing, molecular imaging, diagnosis and therapies, based on their exceptionally diverse properties. Properties such as size, morphology, aggregation propensity, functionalization, biocompatibility, and biodegradability are being continuously optimized to maximize their biomedical efficacy. Among these nanostructures, nanocapsules are the next-generation approach promising to control "cellular health," by programming the release of cargo to the target cell or organ based on endogenous (e.g. enzymes and pH) or external (light, magnetic field, ultrasound) stimuli. Nanocapsules are a class of porous polymeric or inorganic particles in the nanometer range, with tunable features such as pore diameter, pore topology, diverse surface properties, and loading efficiencies that can be easily tailored to several biomedical applications. However, the use of nanocapsules in the biomedicine field is still in its infancy. Most studies have been performed in silico or in cell lines, and just very few studies have been done in animal models of disease. This chapter focuses on those studies where nanocapsules have been well characterized in vitro and in vivo, with the potential to be translated into the clinic. Moreover, this chapter also offers an overview of the recent advances in nanocapsule-based approaches for cancer therapy.
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    NanoKeepers: Stimuli responsive nanocapsules for programmed specific targeting and drug delivery
    (Royal Soc Chemistry, 2014) Hernandez, Frank J.; Hernandez, Luiza I.; Kavruk, Murat; Arıca, Yakup M.; Bayramoğlu, Gülay; Borsa, Barış Ata; Özalp, Veli Cengiz
    Bacterial resistance is a high priority clinical issue worldwide. Thus, an effective system that rapidly provides specific treatment for bacterial infections using controlled dose release remains an unmet clinical need. Herein, we report on the Nano Keepers approach for the specific targeting of S. aureus with controlled release of antibiotics based on nuclease activity.
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    Nuclease activity as a specific biomarker for breast cancer
    (Royal Soc Chemistry, 2016) Hernandez, Luiza I.; Cengiz Özalp, Veli; Hernandez, Frank J.
    We report on the activity of nucleases derived from cancer cells as a means for specific targeting using nucleic acid probes (substrates). We hypothesize that cancer cells can be differentiated from healthy cells based on their nuclease activity profile, and thus, any method based on this property represents a novel alternative for diagnostic and therapeutic intervention.
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    Small molecule detection by lateral flow strips via aptamer-gated silica nanoprobes
    (Royal Soc Chemistry, 2016) Özalp, V. Cengiz; Cam, Dilek; Hernandez, Frank J.; Hernandez, Luiza I.; Schafer, Thomas; Öktem, Hüseyin A.
    A fast, sensitive and ratiometric biosensor strategy for small molecule detection was developed through nanopore actuation. The new platform engineers together, a highly selective molecular recognition element, aptamers, and a novel signal amplification mechanism, gated nanopores. As a proof of concept, aptamer gated silica nanoparticles have been successfully used as a sensing platform for the detection of ATP concentrations at a wide linear range from 100 mu M up to 2 mM.
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    Staphylococcus aureus detection in blood samples by silica nanoparticle-oligonucleotides conjugates
    (Elsevier Advanced Technology, 2016) Borsa, Barış Ata; Tuna, Bilge G.; Hernandez, Frank J.; Hernandez, Luiza I.; Bayramoğlu, Gülay; Arıca, M. Yakup; Özalp, Veli Cengiz
    A fast, specific and sensitive homogeneous assay for Staphylococcus aureus detection was developed by measuring the activity of secreted nuclease from the bacteria via a modified DNA oligonucleotide. As biosensor format, an effective system, Nanokeepers as previously reported, were used for triggered release of confined fluorophores, and hence specific detection of S. aureus on nuclease activity was obtained. The interference from blood components for fluorescent quantification was eliminated by a pre purification by aptamer-functionalized silica magnetic nanoparticles. The reported assay system was exclusively formed by nucleic acid oligos and magnetic or mesoporous silica nanoparticles, that can be used on blood samples in a stepwise manner. The assay was successfully used as a sensing platform for the specific detection of S. aureus cells as low as 682 CFU in whole blood. (C) 2016 Elsevier B.V. All rights reserved.