Staphylococcus aureus detection in blood samples by silica nanoparticle-oligonucleotides conjugates
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Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Advanced Technology
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A fast, specific and sensitive homogeneous assay for Staphylococcus aureus detection was developed by measuring the activity of secreted nuclease from the bacteria via a modified DNA oligonucleotide. As biosensor format, an effective system, Nanokeepers as previously reported, were used for triggered release of confined fluorophores, and hence specific detection of S. aureus on nuclease activity was obtained. The interference from blood components for fluorescent quantification was eliminated by a pre purification by aptamer-functionalized silica magnetic nanoparticles. The reported assay system was exclusively formed by nucleic acid oligos and magnetic or mesoporous silica nanoparticles, that can be used on blood samples in a stepwise manner. The assay was successfully used as a sensing platform for the specific detection of S. aureus cells as low as 682 CFU in whole blood. (C) 2016 Elsevier B.V. All rights reserved.
Açıklama
Ozalp, Veli Cengiz/0000-0002-7659-5990; Tuna, Bilge Guvenc/0000-0003-1348-1336; Hernandez, Frank J./0000-0001-6308-8087
Anahtar Kelimeler
Micrococcal Nuclease, Staphylococcus Aureus, Aptamers, Nanoparticles, Magnetic Separation
Kaynak
Biosensors & Bioelectronics
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
86
