Pathogen detection by core-shell type aptamer-magnetic preconcentration coupled to real-time PCR

Basit Öğe Kaydı
dc.contributor.authorÖzalp, Veli Cengiz
dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorKavruk, Murat
dc.contributor.authorKeskin, Batuhan B.
dc.contributor.authorÖktem, Hüseyin A.
dc.contributor.authorArıca, M. Yakup
dc.date.accessioned2021-05-15T12:40:48Z
dc.date.available2021-05-15T12:40:48Z
dc.date.issued2014
dc.departmentTıp Fakültesi, Biyokimya Bölümü Anabilim Dalıen_US
dc.descriptionKavruk, Murat/0000-0001-5331-7253; Ozalp, Veli Cengiz/0000-0002-7659-5990
dc.description.abstractThe presence of pathogenic bacteria is a major health risk factor in food samples and the commercial food supply chain is susceptible to bacterial contamination. Thus, rapid and sensitive identification methods are in demand for the food industry. Quantitative polymerase chain reaction (PCR) is one of the reliable specific methods with reasonably fast assay times. However, many constituents in food samples interfere with PCR, resulting in false results and thus hindering the usability of the method. Therefore, we aimed to develop an aptamer-based magnetic separation system as a sample preparation method for subsequent identification and quantification of the contaminant bacteria by real-time PCR. To achieve this goal, magnetic beads were prepared via suspension polymerization and grafted with glycidylmethacrylate (GMA) brushes that were modified into high quantities of amino groups. The magnetic beads were decorated with two different aptamer sequences binding specifically to Escherichia coli or Salmonella typhimurium. The results showed that even 1.0% milk inhibited PCR, but our magnetic affinity system capture of bacteria from 100% milk samples allowed accurate determination of bacterial contamination at less than 2.0 h with limit of detection around 100 CFU/mL for both bacteria in spiked-milk samples. (C) 2013 Elsevier Inc. All rights reserved.en_US
dc.identifier.doi10.1016/j.ab.2013.11.022
dc.identifier.endpage125en_US
dc.identifier.issn0003-2697
dc.identifier.issn1096-0309
dc.identifier.pmid24291643
dc.identifier.scopus2-s2.0-84891544224
dc.identifier.scopusqualityQ2
dc.identifier.startpage119en_US
dc.identifier.urihttps://doi.org/10.1016/j.ab.2013.11.022
dc.identifier.urihttps://hdl.handle.net/20.500.12939/681
dc.identifier.volume447en_US
dc.identifier.wosWOS:000330089500020
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzalp, Veli Cengiz
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofAnalytical Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPathogensen_US
dc.subjectMagnetic Beadsen_US
dc.subjectBiosensorsen_US
dc.subjectAptamersen_US
dc.subjectRTPCRen_US
dc.titlePathogen detection by core-shell type aptamer-magnetic preconcentration coupled to real-time PCR
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Temel Tıp Bilimleri Bölümü Koleksiyonu