In vitro evaluation of pediatric restorative materials on human dental pulp stem cells
[ X ]
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This in vitro study aimed to compare the cellular viability of four different restorative materials on human dental
pulp stem cells (hDPSCs). The necessary tissues of isolated hDPSCs were obtained from 10 impacted third molars
of healthy individuals 19–25 years old undergoing surgical extraction. The effects of GC Fuji IX GP, GC EQUIA
Forte, APA Glass Carbomer, and APA Esthetic Fill eluates on hDPSCs’ viability on the first, third, and seventh
days were examined by flow cytometry using an Annexin V binding assay. The cell viability ratios differed
significantly between the restorative material groups (p < 0.001). When the restorative materials and the control
group were compared, the control group had the highest cell viability on all experiment days, while the Glass
Carbomer group had the highest percentage of viable cells of all the restorative material groups. A significant
difference between days in terms of cellular viability (p < 0.001) was found. Considering the early apoptotic cell
ratios, no significant difference was found between the groups and days (p > 0.05). Furthermore, based on time,
except for the first day of the GC Fuji IX GP group, no statistically significant difference was observed in the early
apoptotic cell ratios in each group (p > 0.05). When the rates of late apoptotic cells were compared between the
groups, no significant difference was observed, except for with the control group, which had the lowest rate of
late apoptotic cells (p < 0.001). However, there was a significant difference in necrotic cell ratios between groups
and days (p < 0.001; p = 0.001). The lowest rates of necrotic cells were found in the control group and Glass
Carbomer groups, while the rates of necrotic cells were similar among the other groups. Therefore, we concluded
that APA Glass Carbomer presents the highest cellular viability among the materials.
Açıklama
Anahtar Kelimeler
Restorative Material, Stem Cell, Human Dental Pulp, Cell Viability, Annexin V
Kaynak
Tissue and Cell
WoS Q Değeri
Q1
Scopus Q Değeri
Q3
Cilt
77
Sayı
Künye
Ilisulu, S. C., Oztop, N. E., Akkoç, T., & Seymen, F. (2022). In vitro evaluation of pediatric restorative materials on human dental pulp stem cells. Tissue and Cell, 77.
