Molecular mechanisms of colistin resistance among klebsiella pneumoniae strains

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dc.contributor.authorBorsa, Barış Ata
dc.contributor.authorDemirci, Mehmet
dc.contributor.authorGüngördü-Dalar, Zeynep
dc.contributor.authorKarabıyık, Göktuğ
dc.contributor.authorAygün, Gökhan
dc.contributor.authorKüçükbasmacı, Ömer
dc.date.accessioned2021-05-15T12:41:23Z
dc.date.available2021-05-15T12:41:23Z
dc.date.issued2019
dc.departmentTıp Fakültesien_US
dc.descriptionDemirci, Mehmet/0000-0001-9670-2426; Karabiyik, Goktug/0000-0002-0396-7626; GUNGORDU DALAR, Zeynep/0000-0003-2177-4235; Kucukbasmaci, Omer/0000-0002-8678-4218; Aygun, Gokhan/0000-0001-6915-9843
dc.description.abstractBackground: The increasing rate of infections caused by multiple drug resistant gram-negative bacteria has led to resuscitation of colistin. As a result, colistin resistance, mainly among Klebsiella pneumoniae strains has also been increased. The aim of this study was to investigate molecular mechanisms behind colistin resistance. Methods: Twenty colistin-resistant K. pneumoniae strains isolated from clinical samples of different patients were involved in this study. VITEK2 automated ID/AST system (Biomeriux, France) was used for the identification and also the susceptibility testing for antibiotics other than colistin. Colistin susceptibility was determined by broth microdilution method. To identify the mechanisms of resistance, mutations on mgrB genes, expression levels of pmrA, pmrB, pmrC, pmrD, pmrE, pmrK, phoQ, and phoP genes, and the presence of plasmid mediated colistin resistance genes, mcr-1 and mcr-2 were investigated. Results: As a result of the study, increased expression levels of the pmrA, pmrB, pmrD, pmrK, phoP, and phoQ genes were observed. All colistin resistant strains were found wild type for the mgrB gene which is thought to be responsible for colistin resistance. Also, no mcr-1 or mcr-2 genes which are the causes of plasmid mediated colistin resistance have been detected in any of the strains. Conclusions: Among the colistin resistant K. pneumoniae strains included in our study, increased expression levels of the genes responsible for cell membrane modifications related with colistin resistance were the most common mechanisms.en_US
dc.description.sponsorshipIstanbul University Scientific Research Projects UnitIstanbul University [57713]en_US
dc.description.sponsorshipThis study was supported by the Istanbul University Scientific Research Projects Unit, Project No. 57713.en_US
dc.identifier.doi10.7754/Clin.Lab.2019.180705
dc.identifier.endpage1130en_US
dc.identifier.issn1433-6510
dc.identifier.issue7en_US
dc.identifier.pmid31307167
dc.identifier.scopus2-s2.0-85069929035
dc.identifier.scopusqualityQ3
dc.identifier.startpage1125en_US
dc.identifier.urihttps://doi.org/10.7754/Clin.Lab.2019.180705
dc.identifier.urihttps://hdl.handle.net/20.500.12939/796
dc.identifier.volume65en_US
dc.identifier.wosWOS:000475700400001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorGüngördü-Dalar, Zeynep
dc.language.isoen
dc.publisherClin Lab Publen_US
dc.relation.ispartofClinical Laboratory
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectColistinen_US
dc.subjectKlebsiella Pneumoniaeen_US
dc.subjectResistanceen_US
dc.titleMolecular mechanisms of colistin resistance among klebsiella pneumoniae strains
dc.typeArticle

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