Methylation, sugar puckering and Z-form status of DNA from a heavy metal-acclimated freshwater Gordonia sp.

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dc.contributor.authorGurbanov, Rafig
dc.contributor.authorTuncer, Sinem
dc.contributor.authorMingu, Sara
dc.contributor.authorSevercan, Feride
dc.contributor.authorGözen, Ayşe Gül
dc.date.accessioned2021-05-15T11:34:22Z
dc.date.available2021-05-15T11:34:22Z
dc.date.issued2019
dc.departmentTıp Fakültesi, Biyofizik Anabilim Dalıen_US
dc.descriptionGozen, Ayse Gul/0000-0001-5479-051X; Tuncer, Sinem/0000-0002-8947-8646; Mingu-Akmete, Sara/0000-0002-7332-0764
dc.description.abstractHeavy metal acclimation of bacteria is of particular interest in many aspects. It could add to our understanding of adaptation strategies applied by bacteria, as well as help us in devising ways to use such adaptive bacteria for bioremediation. In this study, we have explored the changes in the DNA of an aquatic Gordonia sp. acclimated to silver, cadmium, and lead. We have measured the changes in the DNA extracted from the acclimated bacteria by using ATR-FTIR coupled with unsupervised and supervised pattern recognition algorithms. Although whole-cell FTIR studies do reveal nucleic acid changes, the special care should be taken when considering marker nucleic acid bands in such spectra, as various other cell or tissue constituents also yield IR bands in the same region. An FTIR study on isolated DNA can be used to avoid this problem. The IR spectral profiles of the DNA molecules revealed significant changes in the backbone and sugar conformations of upon acclimation. We then further analyzed the DNA's global cytosine-methylation patterns of the heavy metal-acclimated bacteria. We aimed to find out whether epigenetic mechanisms operate in bacteria for survival and growth in inhibitory heavy metal concentrations or not. We found hypermethylation in Cd acclimation but hypomethylation for both Pb and Ag in Gordonia sp. Our results imply that changes in the conformational and methylation states of DNA seem to let bacteria to thrive in otherwise inhibitory conditions and mark the involvement of epigenetic modulation in acclimation processes.en_US
dc.description.sponsorshipMiddle East Technical University Scientific Research Project Coordination Unit [BAP-01-08-2017-003]en_US
dc.description.sponsorshipThis study was supported by the Middle East Technical University Scientific Research Project Coordination Unit (Project No: BAP-01-08-2017-003).en_US
dc.identifier.doi10.1016/j.jphotobiol.2019.111580
dc.identifier.issn1011-1344
dc.identifier.pmid31394353
dc.identifier.scopus2-s2.0-85071784069
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.jphotobiol.2019.111580
dc.identifier.urihttps://hdl.handle.net/20.500.12939/314
dc.identifier.volume198en_US
dc.identifier.wosWOS:000486357300014
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSevercan, Feride
dc.language.isoen
dc.publisherElsevier Science Saen_US
dc.relation.ispartofJournal of Photochemistry and Photobiology B-Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFreshwater Bacteriaen_US
dc.subjectAcclimationen_US
dc.subjectDNA Methylationen_US
dc.subjectDNA Conformationen_US
dc.subjectFTIRen_US
dc.titleMethylation, sugar puckering and Z-form status of DNA from a heavy metal-acclimated freshwater Gordonia sp.
dc.typeArticle

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