Aspects of silver tolerance in bacteria: infrared spectral changes and epigenetic clues

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dc.contributor.authorGurbanov, Rafig
dc.contributor.authorÖzek, Nihal S.
dc.contributor.authorTuncer, Sinem
dc.contributor.authorSevercan, Feride
dc.contributor.authorGözen, Ayşe G.
dc.date.accessioned2021-05-15T12:41:50Z
dc.date.available2021-05-15T12:41:50Z
dc.date.issued2018
dc.departmentTıp Fakültesi, Biyofizik Anabilim Dalıen_US
dc.descriptionGozen, Ayse Gul/0000-0001-5479-051X; Tuncer, Sinem/0000-0002-8947-8646;
dc.description.abstractIn this study, the molecular profile changes leading to the adaptation of bacteria to survive and grow at inhibitory silver concentration were explored. The profile obtained through infrared (IR)-based measurements indicated extensive changes in all biomolecular components, which were supported by chemometric techniques. The changes in biomolecular profile were prominent, including nucleic acids. The changes in nucleic acid region (1350-950 cm(-1)) were encountered as a clue for conformational change in DNA. Further analysis of DNA by IR spectroscopy revealed changes in the backbone and sugar conformations. Moreover, Enzyme-Linked Immunosorbent Assay-based measurements of DNA methylation levels were performed to see if epigenetic mechanisms are in operation during bacterial adaptation to this environmental challenge. The results indicated a notable demethylation in Escherichia coli and methylation in Staphylococcus aureus likely to be associated with their elaborate adaptation process to sustain survival and growth.en_US
dc.description.sponsorshipTubitakTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113Y515]en_US
dc.description.sponsorshipTubitak, Grant/Award number: 113Y515en_US
dc.identifier.doi10.1002/jbio.201700252
dc.identifier.issn1864-063X
dc.identifier.issn1864-0648
dc.identifier.issue5en_US
dc.identifier.pmid29243883
dc.identifier.scopus2-s2.0-85041138808
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/jbio.201700252
dc.identifier.urihttps://hdl.handle.net/20.500.12939/859
dc.identifier.volume11en_US
dc.identifier.wosWOS:000432008800009
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSevercan, Feride
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofJournal of Biophotonics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBacteriaen_US
dc.subjectFTIRen_US
dc.subjectMethylationen_US
dc.subjectSilveren_US
dc.titleAspects of silver tolerance in bacteria: infrared spectral changes and epigenetic clues
dc.typeArticle

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