Prevalence and antimicrobial susceptibilities of anaerobic bacteria isolated from perforated corneal ulcers by culture and multiplex PCR: An evaluation in cases with keratitis and endophthalmitis

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dc.contributor.authorTokman, Hrisi Bahar
dc.contributor.authorİskeleli, Güzin
dc.contributor.authorDalar, Zeynep Güngördü
dc.contributor.authorKangaba, Achille Aime
dc.contributor.authorDemirci, Mehmet
dc.contributor.authorAkay, Hatice K.
dc.contributor.authorKiraz, Nuri
dc.contributor.authorBorsa, Barış Ata
dc.date.accessioned2021-05-15T12:40:51Z
dc.date.available2021-05-15T12:40:51Z
dc.date.issued2014
dc.departmentTıp Fakültesi, Mikrobiyoloji Anabilim Dalıen_US
dc.descriptionDemirci, Mehmet/0000-0001-9670-2426; DALAR, Zeynep GUNGORDU/0000-0003-2177-4235; Ozalp, Veli Cengiz/0000-0002-7659-5990; Tokman, Hrisi Bahar/0000-0002-2205-5120; Kocazeybek, Bekir S/0000-0003-1072-3846
dc.description.abstractBackground: Anaerobic bacteria play an important role in eye infections; however, there is limited epidemiologic data based on the the role of these bacteria in the etiology of keratitis and endophthalmitis. The aim of this research is to determine the prevalence of anaerobic bacteria in perforated corneal ulcers of patients with keratitis and endophthalmitis and to evaluate their antimicrobial susceptibilities. Methods: Corneal scrapings were taken by the ophthalmologist using sterile needles. For the isolation of anaerobic bacteria, samples were inoculated on specific media and were incubated under anaerobic conditions obtained with Anaero-Gen (Oxoid & Mitsubishi Gas Company) in anaerobic jars (Oxoid USA, Inc. Columbia, MD, USA). The molecular identification of anaerobic bacteria was performed by multiplex PCR and the susceptibilities of anaerobic bacteria to penicillin, chloramphenicol, and clindamycin were determined with the E test (bioMerieux). Results: 51 strains of anaerobic bacteria belonging to four different genuses were detected by multiplex PCR and only 46 strains were isolated by culture. All of them were found susceptible to chloramphenicol whereas penicillin resistance was found in 13.3% of P.anaerobius strains, clindamycin resistance was found in 34.8% of P.acnes and 13.3% of P.anaerobius strains. Additionnaly, one strain of P.granulosum was found resistant to clindamycin, one strain of B.fragilis and one strain of P.melaninogenica were found resistant to penicillin and clindamycin. Conclusions: Routine analyses of anaerobes in perforated corneal ulcers is inevitable and usage of appropriate molecular methods, for the detection of bacteria responsible from severe infections which might not be determined by cultivation, may serve for the early decision of the appropriate treatment. Taking into account the increasing antimicrobial resistance of anaerobic bacteria, alternative eye specific antibiotics effective against anaerobes are needed to achieve a successful treatment.en_US
dc.identifier.doi10.7754/Clin.Lab.2014.131113
dc.identifier.endpage1886en_US
dc.identifier.issn1433-6510
dc.identifier.issue11en_US
dc.identifier.pmid25648030
dc.identifier.scopus2-s2.0-84914174449
dc.identifier.scopusqualityQ3
dc.identifier.startpage1879en_US
dc.identifier.urihttps://doi.org/10.7754/Clin.Lab.2014.131113
dc.identifier.urihttps://hdl.handle.net/20.500.12939/694
dc.identifier.volume60en_US
dc.identifier.wosWOS:000344911700014
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorBorsa, Barış Ata
dc.language.isoen
dc.publisherClin Lab Publen_US
dc.relation.ispartofClinical Laboratory
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnaerobic Bacteriaen_US
dc.subjectCorneal Ulcersen_US
dc.subjectKeratitisen_US
dc.subjectEndophthalmitisen_US
dc.subjectCultureen_US
dc.subjectPCRen_US
dc.titlePrevalence and antimicrobial susceptibilities of anaerobic bacteria isolated from perforated corneal ulcers by culture and multiplex PCR: An evaluation in cases with keratitis and endophthalmitis
dc.typeArticle

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