A comparative analysis of treatment-related changes in the diagnostic biomarker active metalloproteinase-8 levels in patients with periodontitis
| dc.contributor.author | Keskin, Mutlu | |
| dc.contributor.author | Rintamarttunen, Juulia | |
| dc.contributor.author | Gülçiçek, Emre | |
| dc.contributor.author | Räisänen, Ismo T. | |
| dc.contributor.author | Gupta, Shipra | |
| dc.contributor.author | Tervahartiala, Taina | |
| dc.contributor.author | Pätilä, Tommi | |
| dc.contributor.author | Sorsa, Timo | |
| dc.date.accessioned | 2023-03-16T08:02:24Z | |
| dc.date.available | 2023-03-16T08:02:24Z | |
| dc.date.issued | 2023 | en_US |
| dc.department | Meslek Yüksekokulları, Sağlık Hizmetleri Meslek Yüksekokulu, Ağız ve Diş Sağlığı Programı | en_US |
| dc.description.abstract | Background: Previous studies have revealed the potential diagnostic utility of aMMP-8, an active form of MMP-8, in periodontal and peri-implant diseases. While non-invasive point-of-care (PoC) chairside aMMP-8 tests have shown promise in this regard, there is a dearth of literature on the evaluation of treatment response using these tests. The present study aimed to investigate treatment-related changes in aMMP-8 levels in individuals with Stage III/IV-Grade C periodontitis compared to a healthy control group, using a quantitative chairside PoC aMMP-8 test, and to determine its correlation with clinical parameters. Methods: The study included 27 adult patients (13 smoker, 14 non-smoker) with stage III/IV-grade C periodontitis and 25 healthy adult subjects. Clinical periodontal measurements, real-time PoC aMMP-8, IFMA aMMP-8, and Western immunoblot analyses were performed before and 1 month after anti-infective scaling and root planing periodontal treatment. Time 0 measurements were taken from the healthy control group to test the consistency of the diagnostic test. Results: Both PoC aMMP-8 and IFMA aMMP-8 tests showed a statistically significant decrease in aMMP-8 levels and improvement in periodontal clinical parameters following treatment (p < 0.05). The PoC aMMP-8 test had high diagnostic sensitivity (85.2%) and specificity (100.0%) for periodontitis and was not affected by smoking (p > 0.05). Treatment also reduced MMP-8 immunoreactivity and activation as demonstrated by Western immunoblot analysis. Conclusion: The PoC aMMP-8 test shows promise as a useful tool for the real-time diagnosis and monitoring of periodontal therapy. | en_US |
| dc.identifier.citation | Keskin, M., Rintamarttunen, J., Gülçiçek, E., Räisänen, I. T., Gupta, S., Tervahartiala, T., Pätilä, T. & Sorsa, T. (2023). A comparative analysis of treatment-related changes in the diagnostic biomarker active metalloproteinase-8 levels in patients with periodontitis. Diagnostics, 13(5), 903. | en_US |
| dc.identifier.issn | 2075-4418 | |
| dc.identifier.issn | 2075-4418 | |
| dc.identifier.issue | 5 | en_US |
| dc.identifier.scopus | 2-s2.0-85149724369 | |
| dc.identifier.scopusquality | Q2 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12939/3439 | |
| dc.identifier.volume | 13 | en_US |
| dc.identifier.wos | WOS:000948143300001 | |
| dc.identifier.wosquality | Q1 | |
| dc.indekslendigikaynak | Web of Science | |
| dc.indekslendigikaynak | Scopus | |
| dc.indekslendigikaynak | PubMed | |
| dc.institutionauthor | Keskin, Mutlu | |
| dc.language.iso | en | |
| dc.relation.ispartof | Diagnostics | |
| dc.relation.isversionof | 10.3390/diagnostics13050903 | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Diagnosis | en_US |
| dc.subject | Inflammation | en_US |
| dc.subject | Matrix Metalloproteinase(s) | en_US |
| dc.subject | Periodontal Diseases | en_US |
| dc.subject | Periodontitis | en_US |
| dc.title | A comparative analysis of treatment-related changes in the diagnostic biomarker active metalloproteinase-8 levels in patients with periodontitis | |
| dc.type | Article |
