Design of a core-shell type immuno-magnetic separation system and multiplex PCR for rapid detection of pathogens from food samples

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dc.contributor.authorÖzalp, V. Cengiz
dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorArica, M. Yakup
dc.contributor.authorOktem, H. Avni
dc.date.accessioned2021-05-15T12:40:57Z
dc.date.available2021-05-15T12:40:57Z
dc.date.issued2013
dc.departmentTıp Fakültesien_US
dc.descriptionOzalp, Veli Cengiz/0000-0002-7659-5990
dc.description.abstractWe report an immuno-magnetic separation system developed by the immobilization of pathogen-specific antibodies on the core-shell magnetic beads. The magnetic beads were grafted with glycidylmethacrylate (GMA) using surface-initiated atom transfer radical polymerization (SI-ATRP). For immuno-magnetic separation (IMS) of target bacterial cells from others, antibodies for Escherichia coli and Salmonella enterica serovar Typhimurium cells were immobilized on the magnetic beads via glutaraldehyde coupling reaction. Our IMS system successfully separated Salmonella cells when the concentrations of target (i.e., Salmonella) and interfering (i.e., E. coli) cells were at the same level. Polymerase chain reaction (PCR) assays amplifying the rfb/rfbE region of the E. coli genome and a 647-bp fragment of the invA region of Salmonella were performed as the specific selection to accurately confirm the presence of E. coli and Salmonella, respectively. IMS and multiplex PCR methods can be used for specific and quantitative detection of pathogens from food samples. Thus, this study developed a reliable and direct system for rapid detection of Salmonella and E. coli in food samples. In addition, IMS method could be easily adapted to detect other pathogens by selecting the pertinent antibody.en_US
dc.identifier.doi10.1007/s00253-013-5231-0
dc.identifier.endpage9551en_US
dc.identifier.issn0175-7598
dc.identifier.issn1432-0614
dc.identifier.issue21en_US
dc.identifier.pmid24048640
dc.identifier.scopus2-s2.0-84885960052
dc.identifier.scopusqualityQ1
dc.identifier.startpage9541en_US
dc.identifier.urihttps://doi.org/10.1007/s00253-013-5231-0
dc.identifier.urihttps://hdl.handle.net/20.500.12939/716
dc.identifier.volume97en_US
dc.identifier.wosWOS:000325617900023
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzalp, V. Cengiz
dc.language.isoen
dc.publisherSpringeren_US
dc.relation.ispartofApplied Microbiology and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectImmuno-Magnetic Separationen_US
dc.subjectMultiplex PCRen_US
dc.subjectFood-Borne Diseasesen_US
dc.subjectSalmonellaen_US
dc.subjectEscherichia Colien_US
dc.titleDesign of a core-shell type immuno-magnetic separation system and multiplex PCR for rapid detection of pathogens from food samples
dc.typeArticle

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