FTIR imaging of structural changes in visceral and subcutaneous adiposity and brown to white adipocyte transdifferentiation

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dc.contributor.authorBaloğlu, Fatma Küçük
dc.contributor.authorGarip, Şebnem
dc.contributor.authorHeise, Sebastian
dc.contributor.authorBrockmann, Gudrun
dc.contributor.authorSevercan, Feride
dc.date.accessioned2021-05-15T12:40:37Z
dc.date.available2021-05-15T12:40:37Z
dc.date.issued2015
dc.departmentTıp Fakültesien_US
dc.description.abstractObesity is a heterogeneous disorder which increases risks for multiple metabolic diseases, such as type 2 diabetes. The current study aims to characterize and compare visceral and subcutaneous adipose tissues in terms of macromolecular content and investigate transdifferentiation between white and brown adipocytes. Regarding this aim, Fourier transform infrared (FTIR) microspectroscopy and uncoupling protein 1 (UCP1) immunohistological staining were used to investigate gonadal (visceral) and inguinal (subcutaneous) adipose tissues of male Berlin fat mice inbred (BFMI) lines, which are spontaneously obese. The results indicated a remarkable increase in the lipid/protein ratio, accompanied with a decrease of UCP1 protein content which might be due to the transdifferentiation of brown adipocytes to white adipocytes in obese groups. It has been widely reported that brown adipose tissue has a strong effect on fatty acid and glucose homeostasis and it could provide an opportunity for the therapy of obesity. When the amount of brown adipose tissue was decreased, lower unsaturation/saturation ratio, qualitatively longer hydrocarbon acyl chain length of lipids and higher amount of triglycerides were obtained in both adipose tissues of mice lines. The results also revealed that subcutaneous adipose tissue was more prone to obesity-induced structural changes than visceral adipose tissue, which could originate from it possessing a lower amount of brown adipose tissue. The current study clearly revealed the power of FTIR microspectroscopy in the precise determination of obesity-induced structural and functional changes in inguinal and gonadal adipose tissue of mice lines.en_US
dc.description.sponsorshipTechnological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [SBAG-110S235]en_US
dc.description.sponsorshipThis work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) with a grant number SBAG-110S235. This work was carried out as a part of the COST Action (BM0901)-SYSGENET.en_US
dc.identifier.doi10.1039/c4an02008a
dc.identifier.endpage2214en_US
dc.identifier.issn0003-2654
dc.identifier.issn1364-5528
dc.identifier.issue7en_US
dc.identifier.pmid25692183
dc.identifier.scopus2-s2.0-84925324701
dc.identifier.scopusqualityQ1
dc.identifier.startpage2205en_US
dc.identifier.urihttps://doi.org/10.1039/c4an02008a
dc.identifier.urihttps://hdl.handle.net/20.500.12939/636
dc.identifier.volume140en_US
dc.identifier.wosWOS:000351405900019
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorGarip, Şebnem
dc.language.isoen
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofAnalyst
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFTIRen_US
dc.titleFTIR imaging of structural changes in visceral and subcutaneous adiposity and brown to white adipocyte transdifferentiation
dc.typeArticle

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