Lipases for targeted industrial applications, focusing on the development of biotechnologically significant aspects: A comprehensive review of recent trends in protein engineering

Basit Öğe Kaydı
dc.contributor.authorVardar-Yel, Nurcan
dc.contributor.authorTütüncü, Havva Esra
dc.contributor.authorSürmeli, Yusuf
dc.date.accessioned2024-06-24T11:48:00Z
dc.date.available2024-06-24T11:48:00Z
dc.date.issued2024en_US
dc.departmentMeslek Yüksekokulları, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Laboratuvar Teknikleri Programıen_US
dc.description.abstractLipases are remarkable biocatalysts, adept at catalyzing the breakdown of diverse compounds into glycerol, fatty acids, and mono- and di-glycerides via hydrolysis. Beyond this, they facilitate esterification, transesterification, alcoholysis, acidolysis, and more, making them versatile in industrial applications. In industrial processes, lipases that exhibit high stability are favored as they can withstand harsh conditions. However, most native lipases are unable to endure adverse conditions, making them unsuitable for industrial use. Protein engineering proves to be a potent technology in the development of lipases that can function effectively under challenging conditions and fulfill criteria for various industrial processes. This review concentrated on new trends in protein engineering to enhance the diversity of lipase genes and employed in silico methods for predicting and comprehensively analyzing target mutations in lipases. Additionally, key molecular factors associated with industrial characteristics of lipases, including thermostability, solvent tolerance, catalytic activity, and substrate preference have been elucidated. The present review delved into how industrial traits can be enhanced through directed evolution (epPCR, gene shuffling), rational design (FRESCO, ASR), combined engineering strategies (i.e. CAST, ISM, and FRISM) as protein engineering methodologies in contexts of biodiesel production, food processing, and applications of detergent, pharmaceutics, and plastic degradation.en_US
dc.identifier.citationVardar-Yel, N., Tütüncü, H. E., Sürmeli, Y. (2024). Lipases for targeted industrial applications, focusing on the development of biotechnologically significant aspects: A comprehensive review of recent trends in protein engineering. International Journal of Biological Macromolecules, 273(Pt 1). 10.1016/j.ijbiomac.2024.132853en_US
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.issuePt 1en_US
dc.identifier.scopus2-s2.0-85195461683
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://hdl.handle.net/20.500.12939/4726
dc.identifier.volume273en_US
dc.identifier.wosWOS:001261090700001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorVardar-Yel, Nurcan
dc.language.isoen
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.relation.isversionof10.1016/j.ijbiomac.2024.132853en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEngineering lipaseen_US
dc.subjectIndustrial applicationen_US
dc.subjectRecent trends in protein engineeringen_US
dc.titleLipases for targeted industrial applications, focusing on the development of biotechnologically significant aspects: A comprehensive review of recent trends in protein engineering
dc.typeReview Article

Dosyalar

Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

PubMed İndeksli Yayınlar Koleksiyonu
Sağlık Hizmetleri Meslek Yüksekokulu Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu