Aptamers: Molecular tools for medical diagnosis

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dc.contributor.authorÖzalp, Veli Cengiz
dc.contributor.authorKavruk, Murat
dc.contributor.authorDilek, Özlem
dc.contributor.authorBayraç, Abdullah Tahir
dc.date.accessioned2021-05-15T12:40:39Z
dc.date.available2021-05-15T12:40:39Z
dc.date.issued2015
dc.departmentTıp Fakültesien_US
dc.descriptionBayrac, Abdullah Tahir T/0000-0002-8049-7867; Ozalp, Veli Cengiz/0000-0002-7659-5990; Kavruk, Murat/0000-0001-5331-7253
dc.description.abstractAptamers have been increasingly applied in biomedical field as a class of biorecognition elements that possess many advantages such as high specificity and binding affinity, easy synthesis, easy modification, small size, non-toxicity and good stability. Many diseases like cancer exhibit cellular aberrations at morphological and molecular levels. Medical diagnosis based on molecular features can be highly specific and extremely sensitive when proper recognition molecule and an efficient signal transduction system are employed. However, bioanalysis of human diseases at the molecular level is an extremely challenging field because effective probes to identify and recognize biomarkers of diseases are not readily available. Traditional bio-recognition molecule, antibody has been exploited to develop excellent diagnosis assays in many formats, but antibodies are insufficient to match the requirements of fast and portable biosensors for point-of-care applications, which are at high demand in pathogenic bacteria detection as well as other diseases like cancer. Aptamers are short single-stranded oligonucleotides, which can be selected from random combinatorial library by SELEX in vitro. This relatively new biorecognition agent has superior intrinsic characteristics for biosensor development. In this review, we first present major aptamer selection technologies and the main formats of biosensors, which were frequently employed in aptasensor development. Then, the current state of aptamers as applied to medical diagnosis was discussed for specifically cancer and pathogen diagnosis. Finally, an overview of aptamer-nanomaterials conjugates was presented in many applications such as diagnosis, bioimaging, and theranostics.en_US
dc.description.sponsorshipTUBITAK ProjectTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [213M315]en_US
dc.description.sponsorshipThis review has been written as part of the TUBITAK Project 213M315.en_US
dc.identifier.doi10.2174/1568026615666150413154233
dc.identifier.endpage1137en_US
dc.identifier.issn1568-0266
dc.identifier.issn1873-5294
dc.identifier.issue12en_US
dc.identifier.pmid25866272
dc.identifier.scopus2-s2.0-84988601042
dc.identifier.scopusqualityQ2
dc.identifier.startpage1125en_US
dc.identifier.urihttps://doi.org/10.2174/1568026615666150413154233
dc.identifier.urihttps://hdl.handle.net/20.500.12939/642
dc.identifier.volume15en_US
dc.identifier.wosWOS:000353246400006
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzalp, Veli Cengiz
dc.language.isoen
dc.publisherBentham Science Publ Ltden_US
dc.relation.ispartofCurrent Topics in Medicinal Chemistry
dc.relation.publicationcategoryDiğeren_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAptamersen_US
dc.subjectBiosensorsen_US
dc.subjectCanceren_US
dc.subjectMedical Diagnosisen_US
dc.subjectPathogen Detectionen_US
dc.subjectSOMAmersen_US
dc.subjectTheranosticen_US
dc.titleAptamers: Molecular tools for medical diagnosis
dc.typeReview Article

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