Magnetic silica nanoparticle-Taq polymerase hybrids for multiple uses in polymerase chain reaction
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Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Royal Soc Chemistry
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Enzyme-magnetic nanoparticle hybrids have been employed as a strategy to improve the biocatalytic usability of biological molecules. For this purpose, we synthesized magnetic core-silica shell nanoparticles for direct one-step easy immobilization of Taq polymerase from a crude extract preparation. The hybrid magnetic particles were synthesized by a magnetic co-precipitation method. The magnetic particles were then coated with a 3-(triethoxysilyl)-propylamine (APTES)/tetraethoxysilane (TEOS) mixture, and the pendant -NH2 groups subsequently functionalized with glutaraldehyde for simultaneous immobilization of Taq-polymerase. The magnetic properties of the particles contributed to fast purification to eliminate inhibitory elements present in the crude extract during Taq polymerase isolation. The Taq-silica hybrid material performed with a similar efficiency to the solution-phase enzyme. Additionally, the new hybrid material allowed reuse of the enzyme multiple times. The silica-Taq polymerase hybrid lost 16% of its activity after 6 cycles. Most importantly, the silica microparticle immobilization extended the functional life of Taq polymerase at room temperature by facilitating a fast cleaning procedure.
Açıklama
Ozalp, Veli Cengiz/0000-0002-7659-5990
Anahtar Kelimeler
Magnetic Silica, Nanoparticle-Taq Polymerase Hybrids
Kaynak
Rsc Advances
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
5
Sayı
106
