Magnetic silica nanoparticle-Taq polymerase hybrids for multiple uses in polymerase chain reaction

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dc.contributor.authorÖzalp, V. Cengiz
dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorArıca, M. Yakup
dc.date.accessioned2021-05-15T12:40:36Z
dc.date.available2021-05-15T12:40:36Z
dc.date.issued2015
dc.departmentTıp Fakültesien_US
dc.descriptionOzalp, Veli Cengiz/0000-0002-7659-5990
dc.description.abstractEnzyme-magnetic nanoparticle hybrids have been employed as a strategy to improve the biocatalytic usability of biological molecules. For this purpose, we synthesized magnetic core-silica shell nanoparticles for direct one-step easy immobilization of Taq polymerase from a crude extract preparation. The hybrid magnetic particles were synthesized by a magnetic co-precipitation method. The magnetic particles were then coated with a 3-(triethoxysilyl)-propylamine (APTES)/tetraethoxysilane (TEOS) mixture, and the pendant -NH2 groups subsequently functionalized with glutaraldehyde for simultaneous immobilization of Taq-polymerase. The magnetic properties of the particles contributed to fast purification to eliminate inhibitory elements present in the crude extract during Taq polymerase isolation. The Taq-silica hybrid material performed with a similar efficiency to the solution-phase enzyme. Additionally, the new hybrid material allowed reuse of the enzyme multiple times. The silica-Taq polymerase hybrid lost 16% of its activity after 6 cycles. Most importantly, the silica microparticle immobilization extended the functional life of Taq polymerase at room temperature by facilitating a fast cleaning procedure.en_US
dc.description.sponsorshipTUBITAK 1001 projectTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [213M315]en_US
dc.description.sponsorshipThis work was partially supported by TUBITAK 1001 project no.: 213M315.en_US
dc.identifier.doi10.1039/c5ra15677g
dc.identifier.endpage87678en_US
dc.identifier.issn2046-2069
dc.identifier.issue106en_US
dc.identifier.scopus2-s2.0-84945312264
dc.identifier.scopusqualityQ1
dc.identifier.startpage87672en_US
dc.identifier.urihttps://doi.org/10.1039/c5ra15677g
dc.identifier.urihttps://hdl.handle.net/20.500.12939/627
dc.identifier.volume5en_US
dc.identifier.wosWOS:000363233500094
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorÖzalp, V. Cengiz
dc.language.isoen
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofRsc Advances
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMagnetic Silicaen_US
dc.subjectNanoparticle-Taq Polymerase Hybridsen_US
dc.titleMagnetic silica nanoparticle-Taq polymerase hybrids for multiple uses in polymerase chain reaction
dc.typeArticle

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